Source

Rabbit

Reactivity

Human

Background

Na+/H+ exchanger regulatory factor is one of several related PDZ domain-containing proteins. NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3. NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains, and with the ERM family of proteins, including merlin, via its carboxy-terminus. NHERF1 may play an important role in breast cancer. Estrogen has been found to induce NHERF1 in estrogen receptor-positive breast cancer cells. Furthermore, NHERF1 has been shown to bind to PDGFR, which is activated in breast carcinomas. NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1, and PDGFR. Therefore, NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of PI3K/Akt signaling in normal cells; this mechanism may be disrupted in cancer. NHERF1 also binds to the cystic fibrosis transmembrane conductance regulator, which functions as an ion channel and has disease-causing mutations in cystic fibrosis. Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting, and trafficking.

References

[1] Donowitz, M. et al. (2005) J Physiol 567, 3-11.
[2] Voltz, J.W. et al. (2001) Oncogene 20, 6309-14.
[3] Stemmer-Rachamimov, A.O. et al. (2001) Am J Pathol 158, 57-62.
[4] Takahashi, Y. et al. (2006) EMBO J 25, 910-20.
[5] Wheeler, D. et al. (2007) J Biol Chem 282, 25076-87.
[6] Weinman, E.J. et al. (2000) Am J Physiol Renal Physiol 279, F393-9.

Raw materials

Preparation Products