?????
|
|
????? ??
- ?? ??
- -20°C
- ??? ??
- ??, ???? ??
- ??
- ???
- ???? ??
- bovine intestine
- Specific Activity
- ≥20units/mg protein
- CAS ??????
- 9014-74-8
??
- ?? ? ?? ??
- ?? ? ???? ?? (GHS)
| WGK ?? | 3 | ||
|---|---|---|---|
| TSCA | TSCA listed | ||
| ???? ??? | 11 - Combustible Solids |
| ????(GHS): |
 
|
|||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ?? ?: | Danger | |||||||||||||||||||||||||||||||||||
| ??·?? ??: |
|
|||||||||||||||||||||||||||||||||||
| ??????: |
|
????? C??? ??, ??, ??
??
Typical conditions for fusion protein cleavage:Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween 20
Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mix
Incubate reaction mixture at ~25 °C for 16 hours
??
An enzyme found in the small intestine, which converts trypsinogen into trypsin.?? ??
Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.????? ?? ?? ? ???
???
?? ??
????? ?? ??
???( 188)?? ??
| ??? | ?? | ??? | ?? | ?? ? | ?? |
|---|---|---|---|---|---|
| Shanghai Zheyan Biotech Co., Ltd. | 18017610038 |
zheyansh@163.com | CHINA | 3619 | 58 |
| BOC Sciences | +1-631-485-4226 |
inquiry@bocsci.com | United States | 19936 | 58 |
| career henan chemical co | +86-0371-86658258 |
factory@coreychem.com | China | 29792 | 58 |
| Creative Enzymes | 1-516-855-7709 |
info@creative-enzymes.com | United States | 8748 | 58 |
| ANHUI WITOP BIOTECH CO., LTD | +8618071025641 |
eric@witopchemical.com | China | 23541 | 58 |
| Wuhan Fortuna Chemical Co., Ltd | +86-27-59207850 |
info@fortunachem.com | China | 6001 | 58 |
| Hebei Fengjia New Material Co., Ltd | +86-0311-87836622 +86-18712993135 |
sales01@tairunfaz.com | China | 8051 | 58 |
| Nanjing Duly Biotech Co.,Ltd | +86-18013301590 |
doris@njduly.com | China | 2448 | 58 |
| Zibo Hangyu Biotechnology Development Co., Ltd | +86-0533-2185556 +86-15965530500 |
nickzhang@hangyubiotech.com | China | 9941 | 58 |
| HANGZHOU LEAP CHEM CO., LTD. | +86-571-87711850 +86-15355479329 |
market18@leapchem.com | China | 43333 | 58 |
????? ?? ??:
??? ???? ????? ??(2,2,6,6-?????-3,5-???????) ???(II) ????????? ?????? ?? ????????? ??????? ?? ???? ????????? ??????? ?2?
Proteinase K
α-Chymotrypsin
METHYL ISOCYANOACETATE
SALCOMINE
Ethyl isocyanoacetate
TERT-BUTYL ISOCYANIDE
1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
PHENYLSELENOL
N-BUTYLISOCYANIDE
Tris(2,4-pentanedionato)chroMiuM(III)