Anti-NF-kB p65 Antibody, Mouse Monoclonal

Mouse

ELISA,FCM,ICC/IF

Human

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RELA / Transcription factor p65 (rh RELA / Transcription factor p65; Catalog#12054-H09E; Q04206-1; Met 1-Tyr 306). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Anti-NF-kB p65 Antibody, Mouse Monoclonal---Cat: 12054-MM04

Immunofluorescence staining of human RELA (NF-KB p65) in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-human RELA monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p5 and its precursor p15), and NF-kappaB2 (p52 and its precursor p1), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation.NF-κB（核因子激活的B細(xì)胞的κ-輕鏈增強(qiáng)）是一種蛋白質(zhì)復(fù)合物，其控制轉(zhuǎn)錄的DNA，細(xì)胞因子產(chǎn)生和細(xì)胞存活。NF-κB幾乎存在于所有動(dòng)物細(xì)胞類型中，并參與細(xì)胞對刺激的反應(yīng)，如應(yīng)激，細(xì)胞因子，自由基，重金屬，紫外線照射，氧化LDL和細(xì)菌或病毒抗原。NF-κB在調(diào)節(jié)對感染的免疫應(yīng)答中起關(guān)鍵作用。NF-κB的不正確調(diào)節(jié)與癌癥，炎癥和自身免疫疾病，感染性休克，病毒感染和免疫發(fā)育不當(dāng)有關(guān)。NF-κB也與突觸可塑性和記憶過程有關(guān)。ANTI-NF-KB P65抗體是一類可以特異性結(jié)合ANTI-NF-KB P65的克隆抗體，主要用于檢測ANTI-NF-KB P65的Western Blot、IHC-P、IF、ELISA、Co-IP等多種免疫學(xué)實(shí)驗(yàn)。