JC-1
| 中文名稱 | JC-1 |
|---|---|
| 中文同義詞 | 5,5',6,6'-四氯-1,1',3,3'-四乙基苯并咪唑羰花青碘化物;線粒體膜電位熒光探針JC-1;5,5',6,6'-四氯-1,1',3,3'-四乙基苯并咪唑羰花菁碘化物;可見光吸收劑515;JC-1(線粒體膜電位探針);CBIC2(3),5,5′,6,6′-TETRACHLORO-1,1′,3,3′-TETRAETHYL-IMIDACARBOCYANINE IODIDE;化合物JC-1;JC-1 氯化物 |
| 英文名稱 | 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide |
| 英文同義詞 | 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide;JC-1,5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide, 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide, CBIC2(3);JC-1 jodide;1h-benzimidazolium, 5,6-dichloro-2[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2h-benzimidazol-2-ylidene)-1-propenyl]-1,3-diethyl-, Iodide;5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-2-benzimidazolinylidene)propenyl]-1,3-diethylbenzimidazolium Iodide;Benzimidazolium, 5,6-dichloro-2-[3-(5,6-dichloro-1,3diethyl-2-benzimidazolinylidene)propenyl]-1,3-diethyl-, Iodide;Benzimidazolocarbocyanine Iodide, 5,50,6,60-tetrachloro-1,10,3,30-tetraethyl-;Imidacarbocyanine Iodide, 1,10,3,30-tetraethyl-5,50,6,60-tetrachloro- |
| CAS號(hào) | 3520-43-2 |
| 分子式 | C25H27Cl4N4.I |
| 分子量 | 652.228 |
| EINECS號(hào) | 200-258-5 |
| 相關(guān)類別 | 微生物Microbiology;小分子抑制劑;生化試劑;化工;Cyanine |
| Mol文件 | 3520-43-2.mol |
| 結(jié)構(gòu)式 |  |
JC-1 性質(zhì)
| 熔點(diǎn) | 275–278 ℃ |
|---|---|
| 儲(chǔ)存條件 | room temp |
| 溶解度 | 溶于甲醇、N,N-二甲基甲酰胺、二甲亞砜 |
| 形態(tài) | 晶體 |
| 顏色 | 深紅色 |
| 外觀 | Solid Powder |
| ε(消光系數(shù)) | ≥175000 at 511-517nm in methanol |
| 最大波長(zhǎng)(λmax) | 514nm |
| 穩(wěn)定性 | 自購(gòu)買之日起，在 -20°C 下按供應(yīng)狀態(tài)干燥保存最多 2 年。 DMSO 或蒸餾水中的溶液可在 4°C 下儲(chǔ)存長(zhǎng)達(dá) 1 年。不要凍結(jié)/解凍。僅通過過濾對(duì)溶液進(jìn)行滅菌，而不是通過高壓滅菌 |
| 生物領(lǐng)域應(yīng)用 | Detectingmitochondrialmembrane potential,ABCB1,ABCC1,and ABCG2 transporters inhibitors,nucleic acid hybridization,prostate cancer; treating cellular death,Alzheimer’s disease; apoptosis assay; cytotoxicity assay; hematotoxicity |
| 主要應(yīng)用 | Langmuir-Blodgett films; lasing systems; nonlinear optical materials; photographic materials1, |
| InChI | InChI=1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1 |
| InChIKey | FYNNIUVBDKICAX-UHFFFAOYSA-M |
| SMILES | C12C=C(C(Cl)=CC=1N(/C(=C\C=C\C1=[N+](CC)C3C=C(Cl)C(Cl)=CC=3N1CC)/N2CC)CC)Cl.[I-] |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Culture cells in 6-, 12- , 24-, or 96-well plates at a density of 5× 10
5
cells/mL. Incubate the cells according to your normal protocol.
2. Ensure that the JC-1 and DMSO has equilibrated to room temperature, and then prepare a 200 μM stock solution by dissolving the contents of one vial in 230 μL of the DMSO provided.
3. For the control tube, allow the vial of CCCP has come to room temperature, add 1 μL of CCCP (50 mM). Incubate cells at 37°C for 5 minutes.
4. Add 10 μL JC-1 (200 μM) per well to make the final concentration at 2 μM. Incubate cells at 37°C, 5% CO
2
, for 15-20 minutes. If additional labeling followed, for example with an annexin V, begin with step 2.a. If not, proceed with step 1.e.
5. After incubation, centrifuge cells for 3-4 minutes at 400× g at 4°C, carefully aspirate the supernant.
6. Wash cells twice with PBS (1×): add 2 mL PBS (1×) to suspend cells and vortex to mix thoroughly. Centrifuge cells for 3-4 minutes at 400× g at 4°C, carefully aspirate the supernant.
7. Add 500 μL PBS (1×) to suspend cells. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
安全信息
| WGK Germany | 3 |
|---|---|
| F | 10-21 |
| 存儲(chǔ)類別 | 11 - 可燃固體 |