熒光標記淀粉樣蛋白肽(1-40)
| 中文名稱 | 熒光標記淀粉樣蛋白肽(1-40) |
|---|---|
| 中文同義詞 | 熒光標記淀粉樣蛋白肽(1-40) |
| 英文名稱 | 5-FAM-Amyloid β-Protein (1-40) |
| 英文同義詞 | 5-FAM-Amyloid β-Protein (1-40);β-Amyloid (1-40), FAM-labeled;5-FAM-Amyloid β-Protein (1-40) trifluoroacetate salt;5-FAM-Amyloid b-Protein (1-40) trifluoroacetate salt;Fam-βAmyloid(1-40) |
| CAS號 | 1678416-08-4 |
| 分子式 | |
| 分子量 | 0 |
| EINECS號 | |
| 相關(guān)類別 | |
| Mol文件 | Mol File |
| 結(jié)構(gòu)式 |
熒光標記淀粉樣蛋白肽(1-40) 性質(zhì)
| 序列 | FAM-Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val |
|---|
λ ex =?492?nm; λ em =?518?nm
Apical-to-basolateral exchange across endothelial monolayer of fluorescent Aβ42 (FAM-labeled Human β-Amyloid (1-42) and Fluor ? 488-labeled β-Amyloid (1-42)) or scramble Aβ42 ( FAM-labeled scrambled β-Amyloid (1-42) ) (1-100 μM) is monitored in transendothelial electrical resistance (TEER) during cell monolayer’s formation over time (over 120?min in presence or absence of Aβ24). Human β-Amyloid (1-24) (1 μM) results in H-Aβ42 retention, thus reducing its efflux through the BBB and therefore preventing an efficient mechanism of Aβ42 clearance.The in vitro BBB model is used to investigate the passage of FAM-labeled or 488-conjugated H-Aβ42 across the endothelial cell monolayer.?The addition of fluorescently labeled H-Aβ42 (FAM-labeled Human β-Amyloid (1-42) or Fluor ? 488-labeled β-Amyloid (1-42)) to the apical side of cell inserts results in effective BBB crossing, FAM-labeled scrambled β-Amyloid (1-42) is not efficiently transcytosed.