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144489-09-8

144489-09-8 Structure

144489-09-8 Structure
IdentificationBack Directory
[Name]

5-Carboxy-DCF DA
[CAS]

144489-09-8
[Synonyms]

5-Carboxy-DCF DA
AJIVVDSJIOOZOJ-UHFFFAOYSA-N
5-Carboxy-2',7'-dichlorofluorescein DA
5-Carboxy-2',7'-dichlorofluorescein diacetate
5-CDCFDA [5-Carboxy-2',7'-dichlorofluorescein Diacetate]
5-Carboxy-2',7'-dichlorofluorescein diacetate, 95%, single isomer
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid, 3',6'-bis(acetyloxy)-2',7'-dichloro-3-oxo-
[Molecular Formula]

C25H14Cl2O9
[MOL File]

144489-09-8.mol
[Molecular Weight]

529.28
Chemical PropertiesBack Directory
[Melting point ]

178-180 °C(Solv: dichloromethane (75-09-2); ethyl acetate (141-78-6))
[Boiling point ]

743.3±60.0 °C(Predicted)
[density ]

1.68±0.1 g/cm3(Predicted)
[solubility ]

Insoluble in water; soluble acetonitrile, chloroform, N,N-dimethylformamide, dimethyl sulfoxide, methanol
[pka]

3.70 ± 0.20, most acidic, temperature: 25°C
[color ]

Light brown solid; Off-white solid
[λmax]

<300nm (Buffer pH4.0); 292nm (MeOH)
[Biological Applications]

Assessing reactive oxygen species (ROS) generation; counting yeast cells; identifying vacuolar system; monitoring blood gas; multidrug resistance-associated protein-2 (MRP2) substrates; studying hepatic disposition; a substrate for measuring esterase activity
Hazard InformationBack Directory
[Description]

5-Carboxy-2',7'-dichlorofluorescein diacetate is a probe that is reactive with primary amines on biomolecules. It diffuses into cells and has been hydrolyzed by intracellular esterases to yield a fluorescent compound.

[Uses]

Non-Activated, Amine-Reactive fluorescent probe
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