| Identification | Back Directory | [Name]
5-TAMRA NHS ester | [CAS]
321862-17-3 | [Synonyms]
5-TAMRA-OSu
5/6-TAMRA-OSu
5-TAMRA NHS ester
TAMRA NHS ester, 5-isomer
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid, 3',6'-bis(dimethylamino)-3-oxo-, 2,5-dioxo-1-pyrrolidinyl ester | [Molecular Formula]
C29H25N3O7 | [MDL Number]
MFCD11858232 | [MOL File]
321862-17-3.mol | [Molecular Weight]
527.52 |
| Chemical Properties | Back Directory | [Boiling point ]
759.4±70.0 °C(Predicted) | [density ]
1.49±0.1 g/cm3(Predicted) | [solubility ]
good in DMF, DMSO, low in water | [pka]
4.06±0.20(Predicted) | [Appearance]
dark colored solid |
| Hazard Information | Back Directory | [Description]
TAMRA (tetramethylrhodamine) is a xanthene dye of rhodamine series. This fluorophore has been used for quite a long time for the preparation of dual-labeled qPCR TaqMan oligonucleotide probes containing TAMRA and fluorescein (FAM).
Like many other xanthene fluorophores, TAMRA is available as two isomers (5- and 6-isomer) with nearly identical optical properties. This product is an isomerically pure 5-TAMRA.
TAMRA NHS is an amine-reactive reagent. It can be used to label proteins, peptides, and modified oligonucleotides containing amine groups. | [Chemical Reactivity]
Primary amines | [Abs/Em Maxima]
541/567 nm | [Fluoresene quantum yield]
0.1 | [Extinction Coefficient]
84000 | [CF260]
0.32 | [CF280]
0.19 | [Spectrally Similar Dyes]
Alexa Fluor? 546, TAMRA, CF? 543, MB? 543 |
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