| Identification | Back Directory | [Name]
DIHYDROFOLATE REDUCTASE F. BOVINE LIVER, ~8 U/MG, 500 UL | [CAS]
9002-03-3 | [Synonyms]
Dihydrofolate Reductase bovine liver
dihydrofolate reductase F. bovine liver
DIHYDROFOLATE REDUCTASEFROM CHICKEN LIVE R
Dihydrofolate Reductase from bovine liver
5,6,7,8-Tetrahydrofolate: NADP+ oxidoreductase
Dihydrofolate Reductase solution from chicken liver
DIHYDROFOLATE REDUCTASE F. BOVINE LIVER, ~8 U/MG, 500 UL | [MDL Number]
MFCD00130955 |
| Hazard Information | Back Directory | [Uses]
Dihydrofolate reductase is a key enzyme in folate metabolism and, therefore, in the production of thymidine. Its role in thymidine biosynthesis is the reduction of dihydrofolate to tetrahydrofolate using the cofactor NADPH[1]. | [Purification Methods]
Dihydrofolate reductase is purified by (NH4)2SO4 precipitation, then fractionation on a Sephadex G-75 column, applied to a Blue Sepharose column and eluted with 1mM dihydrofolate. [Al Rubeai & Dole Biochem J 235 301 1986.] | [References]
[1] Hongnan Cao, et al. The crystal structure of a tetrahydrofolate-bound dihydrofolate reductase reveals the origin of slow product release. Commun Biol. 2018 Dec 12:1:226. DOI:10.1038/s42003-018-0236-y |
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Creative Enzymes
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1-516-855-7709 |
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www.creative-enzymes.com |
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SIGMA-RBI
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800 736 3690 (Orders) |
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www.sigma-aldrich.com |
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