125991-51-7
125991-51-7 結(jié)構(gòu)式
基本信息
中文別名
化合物 T26283化合物 TNP-351
英文別名
TNP 351N-[4-[3-(2,4-Diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)propyl]benzoyl]-L-glutamic acid
L-Glutamic acid, N-[4-[3-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)propyl]benzoyl]-
常見問題列表
生物活性
TNP-351 是一種抗葉酸劑。TNP-351 是一種二氫葉酸還原酶 (DHFR) 抑制劑，在體內(nèi)外對白血病和實(shí)體瘤細(xì)胞均具有有效的抗腫瘤活性。靶點(diǎn)
Antifolate, DHFR
體外研究
TNP-351 inhibits the proliferation of mouse L1210 leukemia cells and human CCRF-CEM lymphoblastic leukemia cells with ED
50
values of 0.79 and 2.7 nM, respectively.
The ED
50
values determined for the parent cell line CCRF-CEM, CCRFCEM R30/6 subline, CCRF-CEM R1, and CCRF-CEM R2 are 2.7, 5.8, 94 and 76 nM, respectively.
Cell Viability Assay
| Cell Line: | L1210 and CCRF-CEM cells |
| Concentration: | 0.1, 0.3, 1, 3, and 10 nM for L1210 cells; 0.3, 1, 3, 10, and 30 for CCRF-CEM cells |
| Incubation Time: | 48 hours for L1210 cells; 72 hours for CCRF-CEM cells |
| Result: | The ED 50 values were 0.79 and 2.7 nM for L1210 and CCRF-CEM cells, respectively. |