1346607-05-3

中文名稱 6-氯-5-(2'-羥基-3'-甲氧基-[1,1'-聯(lián)苯]-4-基)-3-(3-甲氧基苯基)-1H-吡咯并[3,2-D]嘧啶-2,4(3H,5H)-二酮

英文名稱 GSK621

CAS 1346607-05-3

分子式 C26H20ClN3O5

分子量 489.91

MOL 文件 1346607-05-3.mol

更新日期 2024/12/27 11:28:23

1346607-05-3 結(jié)構(gòu)式

基本信息物理化學(xué)性質(zhì) 安全數(shù)據(jù) 圖譜信息 6-氯-5-(2'-羥基-3'-甲氧基-[1,1'-聯(lián)苯]-4-基)-3-(3-甲氧基苯基)-1H-吡咯并[3,2-D]嘧啶-2,4(3H,5H)-二酮價格(試劑級) 常見問題列表

基本信息

中文別名

6-氯-5-(2'-羥基-3'-甲氧基-[1,1'-聯(lián)苯]-4-基)-3-(3-甲氧基苯基)-1H-吡咯并[3,2-D]嘧啶-2,4(3H,5H)-二酮

英文別名

GSK621
GSK621 (GSK 621
GSK621 >=98% (HPLC)
6-Chloro-5-(2'-hydroxy-3'-methoxy-[1,1'-biphenyl]-4-yl)-3-(3-methoxyphenyl)-1H-pyrrolo[3,2-d]pyrimidine-2,4(3H,5H)-dione
1H-Pyrrolo[3,2-d]pyrimidine-2,4(3H,5H)-dione, 6-chloro-5-(2'-hydroxy-3'-methoxy[1,1'-biphenyl]-4-yl)-3-(3-methoxyphenyl)-

所屬類別

醫(yī)藥中間體：雜環(huán)化合物

物理化學(xué)性質(zhì)

密度1.41±0.1 g/cm3(Predicted)

儲存條件Sealed in dry,2-8°C

溶解度insoluble in H2O; insoluble in EtOH; ≥28.5 mg/mL in DMSO

酸度系數(shù)(pKa)9.01±0.40(Predicted)

形態(tài)結(jié)晶固體

顏色Off-white to light yellow

InChI1S/C26H20ClN3O5/c1-34-18-6-3-5-17(13-18)30-25(32)23-20(28-26(30)33)14-22(27)29(23)16-11-9-15(10-12-16)19-7-4-8-21(35-2)24(19)31/h3-14,31H,1-2H3,(H,28,33)

InChIKeyKURYSXLJGKKDHT-UHFFFAOYSA-N

SMILESO=C(NC1=C2N(C3=CC=C(C4=C(O)C(OC)=CC=C4)C=C3)C(Cl)=C1)N(C5=CC=CC(OC)=C5)C2=O

安全數(shù)據(jù)

WGK GermanyWGK 3

存儲類別11 - Combustible Solids

圖譜信息

6-氯-5-(2'-羥基-3'-甲氧基-[1,1'-聯(lián)苯]-4-基)-3-(3-甲氧基苯基)-1H-吡咯并[3,2-D]嘧啶-2,4(3H,5H)-二酮(1346607-05-3)核磁圖(¹HNMR)

常見問題列表

生物活性

GSK621 是特異性的 AMPK 激動劑，其對 AML 細(xì)胞系的IC50 值為13-30 μM。GSK621 可誘導(dǎo)自噬和凋亡。GSK621 可誘導(dǎo)eiF2α 磷酸化 (UPR 激活的一個標(biāo)志)。

體外研究

GSK621 (30 μM) induces AMPKα T172, ACC (S79) and ULK1 (S555) phosphorylation.
GSK621 (30 μM) induces autophagy and apoptosis.
GSK621 treatment also induces PERK phosphorylation, a marker of ER stress, in AML cells.

Cell Proliferation Assay

Cell Line:	MV4-11, OCI-AML3, OCI-AML2, HL-60, Kasumi, HEL, UT7, NB4, TF-1, KG1A, Nomo p28, SKM-1, U937, YHP1, MOLM-14, Mo7e, K562, MOLM-13, EOL-1, SET-2 AML cell lines. 0-30 μM.
Concentration:	0-30 μM.
Incubation Time:	4 d.
Result:	IC ₅₀ values ranged from 13 to 30 μM. Reduced the proliferation of all 20 lines and increased apoptosis in 17 (85%) lines.

Cell Autophagy Assay.

Cell Line:	AML cell lines and primary AML samples.
Concentration:	30 μM.
Incubation Time:	24 h.
Result:	Induced the formation of numerous intracytoplasmic vacuoles including autophagosomes.

體內(nèi)研究

GSK621 (30 mg/kg, ip twice daily) exhibits significant anti-tumor activity in MOLM-14 cells xenograft.