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1513848-14-0

中文名稱 TRFS-GREEN
英文名稱 Carbamic acid, N-(2-butyl-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinolin-6-yl)-, 1,2-dithiolan-4-yl ester
CAS 1513848-14-0
分子式 C20H20N2O4S2
分子量 416.51
MOL 文件 1513848-14-0.mol
更新日期 2026/03/25 16:41:12
1513848-14-0 結(jié)構(gòu)式 1513848-14-0 結(jié)構(gòu)式

基本信息

英文別名
TRFS-green
Carbamic acid, N-(2-butyl-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinolin-6-yl)-, 1,2-dithiolan-4-yl ester

物理化學(xué)性質(zhì)

沸點582.3±50.0 °C(Predicted)
密度1.44±0.1 g/cm3(Predicted)
儲存條件-20°C儲存
溶解度溶于二甲基亞砜
酸度系數(shù)(pKa)11.20±0.20(Predicted)
形態(tài)Solid
顏色White to off-white
TRFS-GREEN價格(試劑級)
報價日期產(chǎn)品編號產(chǎn)品名稱CAS號包裝價格
2025/12/22HY-115640TRFS-green1513848-14-01mg700元
2025/12/22HY-115640TRFS-green1513848-14-05mg2300元
2025/12/22HY-115640TRFS-green1513848-14-010 mM * 1 mLin DMSO2530元

常見問題列表

生物活性
TRFS-green 是用于檢測活細(xì)胞中、硫氧還蛋白還原酶 (TrxR)、且具有高度選擇性的、off?on 熒光探針。TRFS-green的最大吸收波長是 377 nm。
體外研究

TRFS-green itself emits at ~480 nm when excited at 377 nm.
TRFS-green displays a green fluorescence off?on change induced by the TrxR-mediated disulfide cleavage and subsequent intramolecular cyclization to liberate the masked naphthalimide fluorophore.
The fluorescence signal of TRFS-green in living cells was inhibited by pretreating the cells with TrxR inhibitor in a dose-dependent manner, potentiating the development of living cell-based screening assay for identifying TrxR inhibitors.
After incubating the cells with TRFS-green (10 μM) for 4 h, the bright green fluorescence appears.
TRFS-green shows no response to GSH or Cys, the predominant thiols in cells, or the endogenous reducing agents, such as vitamin C and NADPH.
TRFS-green is the first fluorescent probe of TrxR with a green emission. However, the response rate of TRFS-green to the enzyme is slow, and it takes more than 2 h to reach the maximal fluorescence signal even using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent.

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