157134-53-7
中文名稱
DI-8-ANEPPS
英文名稱
DI-8-ANEPPS
CAS
157134-53-7
分子式
C36H52N2O3S
分子量
592.87
MOL 文件
157134-53-7.mol
更新日期
2024/11/26 11:42:17
157134-53-7 結(jié)構(gòu)式
基本信息
中文別名
膜電位熒光探針DI-8-ANEPPSDI-8-ANEPPS(膜電位熒光探針)
英文別名
Jpw 1153DI-8-ANEPPS
Di-8-ANEPPS *CAS#: 157134-53-7*
4-(2-[6-(DIOCTYLAMINO)-2-NAPHTHALENYL]ETHENYL)-1-(3-SULFOPROPYL)PYRIDINIUM INNER SALT
物理化學(xué)性質(zhì)
外觀性狀外觀 : 橘紅色固體
ex/em : 468/633 nm (人工膜)
消光系數(shù)(ε) : 44,000 L?mol?1?cm?1 (甲醇)
ex/em : 468/633 nm (人工膜)
消光系數(shù)(ε) : 44,000 L?mol?1?cm?1 (甲醇)
熔點>200℃
儲存條件2-8°C
溶解度可溶于DMSO
形態(tài)粉末
顏色橙色至紅色
外觀Orange solid
ε(消光系數(shù))≥37 at 495-501 nm in methanol
激發(fā)峰(Ex)/發(fā)射峰(Em)468/633 nm (artificial membrane)
最大波長(λmax)498 nm
生物領(lǐng)域應(yīng)用Measuring membrane potential
常見問題列表
熒光探針
Di-8-ANEPPS 是萘基苯乙烯基（ANEP）電壓敏感染料，它們的熒光激發(fā)和發(fā)射光譜會因電壓的變化而變化。相對于Di-4-ANEPPS，Di-8-ANEPPS有更長的碳鏈尾巴，更容易駐留在細胞膜雙脂層的外層，可勝任長時間的電壓檢測實驗。Di-8-ANEPPS比Di-4-ANEPPS光學(xué)性質(zhì)更穩(wěn)定，光毒性更小。用途
膜電位熒光探針生物活性
Di-8-ANEPPS 是萘基苯乙烯基電壓敏感染料，它們的熒光激發(fā)和發(fā)射光譜會因電壓的變化而變化。體外研究
A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na + -containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the “center” signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse.