1694495-59-4
中文名稱
Alkyne tyramide
英文名稱
Alkyne tyramide
CAS
1694495-59-4
分子式
C13H15NO2
分子量
217.26
MOL 文件
1694495-59-4.mol
更新日期
2023/09/14 14:01:39
1694495-59-4 結(jié)構(gòu)式
基本信息
中文別名
化合物ALKYNE TYRAMIDE 英文別名
Alkyne tyramide4-Pentynamide, N-[2-(4-hydroxyphenyl)ethyl]-
Alkyne tyramide,membrane,mitochondria,topology,Inhibitor,inhibit,APEX2,substrate,yeast
物理化學(xué)性質(zhì)
沸點(diǎn)464.5±35.0 °C(Predicted)
密度1.126±0.06 g/cm3(Predicted)
酸度系數(shù)(pKa)10.01±0.15(Predicted)
形態(tài)Solid
顏色White to off-white
Alkyne tyramide價(jià)格(試劑級(jí))
| 報(bào)價(jià)日期 | 產(chǎn)品編號(hào) | 產(chǎn)品名稱 | CAS號(hào) | 包裝 | 價(jià)格 |
| 2026/06/05 | HY-131442 | Alkyne tyramide Alkyne-phenol | 1694495-59-4 | 5 mg | 333元 |
| 2026/06/05 | HY-131442 | Alkyne tyramide Alkyne tyramide | 1694495-59-4 | 10mM * 1mLin DMSO | 366元 |
| 2026/06/05 | HY-131442 | Alkyne-phenol | 1694495-59-4 | 10 mg | 500元 |
常見(jiàn)問(wèn)題列表
生物活性
Alkyne tyramide 是一種抗壞血酸過(guò)氧化物酶 2 (APEX2) 探針。Alkyne tyramide 顯著提高了完整酵母細(xì)胞的 APEX 標(biāo)記效率，比 APEX2 底物生物素苯酚 (BP) 具有更大的細(xì)胞壁滲透性。Alkyne tyramide 還有助于鑒定 APEX 標(biāo)記位點(diǎn)，明確線粒體蛋白的膜拓?fù)涞姆峙洹?/span>體外研究
Yeast cells expressing Su9-APEX2 are incubated with each probe at 2.5 mM final concentration for half an hour, and the labeling reaction is initiated by the addition of 1 mM H2O2. Alkyne tyramide is the most reactive APEX2 substrate for labeling the yeast proteome.
APEX2-mediated Alkyne tyramide labeling in the yeast mitochondria is more effective at probe concentrations above 1 mM.
Alkyne tyramide enables proteomic profiling in the mitochondrial matrix of intact yeast cells with exceptionally high specificity (94%), and offers higher coverage than the traditional APEX2 substrate biotin-phenol (BP).