山羊抗馬IGG/HRP性質(zhì)、用途與生產(chǎn)工藝
Goat polyclonal Secondary Antibody to Horse IgG - H&L (HRP)
山羊
與馬的重鏈和輕鏈IgG反應(yīng)
HRP
IHC-P ICC ELISA WB
SouthernBiotech
ELISA plate was coated with purified horse IgG. Immunoglobulin was detected with Goat Anti-Equine IgG(H+L)-HRP (SB Cat. No. 6040-05).
Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3). The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
山羊抗馬IGG/HRP是以馬IGG為抗原以山羊?yàn)槊庖咚拗鞯睦备^氧化物酶標(biāo)記免疫蛋白，可以特異性結(jié)合馬IGG，主要用于檢測馬IGG的Western blotting、Immunohistocry、ELISA、IF、IHC-P、IHC-F等生物實(shí)驗(yàn)。
山羊抗馬IGG/HRP
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