DPN I Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.

Allgemeine Beschreibung

Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl₂, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.

DPN I Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

DPN I Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Career Henan Chemica Co	+86-0371-86658258 +8613203830695	laboratory@coreychem.com	China	30225	58
MCE(MedChemExpress)	400-199-8808 18019480960	sales@medchemexpress.cn	China	23918	58
3B Pharmachem (Wuhan) International Co.,Ltd.	821-50328103-801 18930552037	3bsc@sina.com	China	15838	69
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51395	80
Shanghai Kanglang Biotechnology Co., Ltd.	021-61998208 18217752821	sales@klbio.cn	China	9621	58
Wuhan Chemstan Biotechnology Co., Ltd.	027-65317797 15926423062	422450190@qq.com	China	10308	58
Shanghai Yuchun Biotechnology Co., Ltd	021-021-52273538 17321241593	1503472510@qq.com	China	5261	58
Merck KGaA	21-20338288	ordercn@merckgroup.com	China	6038	58
Shanghai Bio-Tech Biotechnology Co., Ltd.	4001683301	order@beyotime.com	China	6588	58
Promega (Beijing) Biotech Co Ltd	400-810-8133 861058256268	info@promega.com.cn	China	3766	58

81295-14-9()Verwandte Suche:

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