ANTI-HEAT SHOCK PROTEIN 25 Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse has been used in:
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- radioimmunoassay (RIA)
- immunocytochemistry
- immunohistofluorescence assay
- immunopurification
Allgemeine Beschreibung
Monoclonal anti-Heat Shock Protein 25 (mouse IgG1) isotype) is derived from the IAP-28 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle. Heat shock (stress) proteins (HSPs) are a group of polypeptides synthesized in response to a wide variety of environmental factors, including biotic and abiotic stressors. These proteins are produced by prokaryotic and eukaryotic cells, and HSPs are among the most conserved molecules in phylogeny. Heat shock protein 25 is encoded by the gene mapped to human chromosome 7q11.23. The encoded protein belongs to the class of small HSPs. Small HSPs aggregate to form characteristic ring-shaped structures called heat shock granules. Small HSPs contains a-crystallin domain” (ACD) C-terminal domain, N-terminal domain and C-terminal extension (CTE).
Biochem/physiol Actions
Small heat shock proteins (HSPs) play a vital role in stress tolerance. They act as molecular chaperones in vitro. Murine Hsp25 averts aggregation of citrate synthase and α-glucosidase. It also inhibits actin polymerization and the amyloid fibril formation of Aβ1-42. Mutations in human sHsps result in myopathies, neuropathies and cataract. The small HSPs have also been implicated in phenomena of acquired thermotolerance and neoplasia.
ANTI-HEAT SHOCK PROTEIN 25 Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
