ALPHA-KETOGLUTARATE DEHYDROGENASE Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

α-Ketoglutarate Dehydrogenase from the porcine heart has been used:

• to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
• to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
• as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons

Allgemeine Beschreibung

α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).

Biochem/physiol Actions

α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease.

ALPHA-KETOGLUTARATE DEHYDROGENASE Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

ALPHA-KETOGLUTARATE DEHYDROGENASE Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Nanjing Dulai Biotechnology Co., Ltd.	025-84699383-8003;025-846993838003-8003 18013301593;18013301590	njduly@126.com	China	3293	55
Shanghai Baoman Biotechnology Co., Ltd.	021-62130998	baomanbio@163.com	China	186	55
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51395	80
Guangzhou Tomums Life Science Co., Ltd.	020-31155029 18902330969	sales@tomums.cn	China	4696	58
Hubei Weideli Chemical Reagent Co.，Ltd.	17771473825 13385289472	Z13385289472@163.com	China	4082	58
Shanghai Yingxin Laboratory Equipment Co., Ltd.	021-59178156 17002132182	3146474380@qq.com	China	10000	58
Shanghai Maclean Biochemical Technology Co., LTD	021-50706066 15221275939	shenlinxing@macklin.cn	China	29680	58
Shanghai Guchen Biotechnology Co., LTD	021-34675735 19147740836	1986399151@qq.com	China	9844	58
Merck KGaA	--	ordercn@merckgroup.com	China	6394	58
Cohesion Biosciences	--	sales@cohesionbio.com	China	6282	58

---

• ALPHA-KETOGLUTARATE DEHYDROGENASE
• A-ketoglutarate dehydrogenase from*porcine heart
• multienzyme 2-oxoglutarate dehydrogenase complex
• α-ketoglutarate dehydrogenase from porcine heart
• MULTIENZYME 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, (1) INCLUDING EC 1.2.4.2
• alpha-Ketoglutarate Dehydrogenase from porcine heart
• α-Ketoglutarate Dehydrogenase, from porcine heart
• Alpha-Ketoglutarate Dehydrogenase Activity Microplate Assay Kit
• α-Ketoglutarate Dehydrogenase
• 9031-02-1
• BioChemical
• General Metabolic Enzymes I-Z
• General Metabolic Enzymes
• Metabolomics