Biotin-16-UTP Chemische Eigenschaften,Einsatz,Produktion Methoden
Beschreibung
Biotin-16-UTP is a biotin-labeled UTP used for in vitro transcription to prepare RNA probes[1]. Biotin-16-UTP is an active substrate for RNA polymerase and can be incorporated by a variety of RNA polymerases (including SP6, T3, and T7 RNA polymerases) to form labeled RNA[2, 3]. Biotin-16-UTP-labeled RNA can be detected by reporter molecules linked to streptavidin, avidin, or anti-biotin antibodies[4, 5]. Biotin-16-UTP can be used for molecular hybridization and RNA-protein interaction studies[6].
Verwenden
Biotin-labeled RNA can be used as a hybridization probe for:
- Northern blots
- Southern blots
- Plaque or colony lifts
- RNase protection experiments
- In situ hybridization
- Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
Allgemeine Beschreibung
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the
in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is
in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Biotin-16-UTP Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
