Oxidase, Diamin Chemische Eigenschaften,Einsatz,Produktion Methoden
S-S?tze Betriebsanweisung:
S22:Staub nicht einatmen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
Verwenden
Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.
Definition
An enzyme occurring in the animal digestive system; it converts histidine to histamine.
Biologische Funktion
Diamine oxidase attacks both diamines and histamine in much the same way that MAO attacks monoamines, forming aldehydes. This
enzyme is inhibited by carbonyl-blocking reagents and produces hydrogen peroxide, supporting the role of pyridoxal phosphate and the
flavin prosthetic groups in the catalytic action of the enzyme. Diamine oxidase is recovered in the supernatant after centrifugation and
removal of particulate matter. It is present in kidneys, intestines, liver, lung, and nervous tissue. It limits the biological effects of histamine
and the polymethylene amines putrescine and cadaverine. It also attacks monoamines, but at a higher substrate concentration.Plasma amine oxidases are in blood plasma of mammals and include spermine oxidase, which deaminates spermine and other polyamines.
Allgemeine Beschreibung
Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper. The molecular mass of the enzyme is found to be 170 kDa. The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A. Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.
Oxidase, Diamin Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
