PCR DIG LABELING MIX Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
PCR DIG Labeling MixPLUS is used for direct labeling of amplification products with digoxigenin (DIG)-deoxyuridine triphosphate (dUTP) in polymerase chain reactions (PCR) and for carryover prevention.The PCR DIG Labeling Mix
plus is 10x concentrated. For each reaction, add 10% of the final volume to the reaction mix.
Allgemeine Beschreibung
The PCR DIG Labeling Mix
PLUS is a mixture of lithium salts of deoxyadenosine triphosphate (dATP), deoxycytosine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP), deoxyuridine triphosphate (dUTP) and digoxigenin (DIG)-11-dUTP. This nucleotide mixture can be added directly to polymerase chain reactions (PCR) and the DIG-labeled nucleotide will be incorporated into the PCR product. The incorporation of dUTP instead of deoxythymidine triphosphate (dTTP) allows the degradation of contaminating PCR products from previous amplification reactions with uracil-DNA glycosylase (UNG), preventing carryover contamination.The incorporated DIG label renders the sensitive detection of the PCR product with anti-DIG-conjugates, for example in the PCR ELISA, DIG-labling, and PCR ELISA, DIG-Detection. The PCR DIG Labeling Mix
PLUS gives comparable signal intensities to the PCR DIG Labeling Mix.
PCR DIG LABELING MIX Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
