NAD+/NADH Cell-Based Assay Kit Chemische Eigenschaften,Einsatz,Produktion Methoden

Beschreibung

Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD⁺, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman’s NAD/NADH Cell-Based Assay Kit provides a colorimetric method for measuring intracellular NAD⁺ and NADH in cultured cells. In this assay, NAD⁺ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.

NAD+/NADH Cell-Based Assay Kit Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

NAD+/NADH Cell-Based Assay Kit Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Shanghai Universal Biotech Co.,Ltd	15921930842 15921930842	yh-wang@univ-bio.com	China	25022	58
amyjet	4006800868;027-59626688 18371453821;18771149750	sales@amyjet.com	China	2917	58
Cayman Chemical Company	--	sales@caymanchem.com	China	6838	58
Shenzhen Campbell Biotechnology Co., Ltd.	--		CHINA	6891	58

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• NAD+/NADH Cell-Based Assay Kit