Dehydratase, Carbonat
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- CAS-Nr.
- 9001-03-0
- Bezeichnung:
- Dehydratase, Carbonat
- Englisch Name:
- EC 4.2.1.1
- Synonyma:
- CARBONIC ANHYDRASE;TDX-02;EC 4.2.1.1;Dehydratase;IUB: 4.2.1.1;CA, HUMAN LIVER;PANCREAS, BOVINE;CARBONIC ANHYDRASE I;Dehydratase, carbote;CARBONATE DEHYDRATASE
- CBNumber:
- CB3317318
- Summenformel:
- NULL
- Molgewicht:
- 0
- MOL-Datei:
- Mol file
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Dehydratase, Carbonat Eigenschaften
- RTECS-Nr.
- MT6446000
- storage temp.
- -20°C
- L?slichkeit
- deionized water: soluble, clear to slightly hazy, colorless (Dissolve vial contents in 0.25mL of deionised water.)
- Aggregatzustand
- powder
- Farbe
- white
- Biologische Quelle
- bovine erythrocytes
- Wasserl?slichkeit
- Soluble in water
- Merck
- 13,1822
- Concentration
- 0.01 mCi/ml
- Specific Activity
- 3-30 μCi/mg
- Solvent
- 0.01M NaH2PO4 (pH 7.2)
Dehydratase, Carbonat Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Carbonic anhydrase from human erythrocytes (HCA) has been used to study the molten-globule state of carbonic anhydrase (CA). Chaperone-like α-crystallin binds to this state of the enzyme and prevents its aggregation. The enzyme from sigma has been used for the analysis of thermodynamic stability of the enzyme. Furthermore, its clinical significance has been evaluated in human non-small cell lung cancer.
Allgemeine Beschreibung
Carbonic Anhydrase is a metalloenzymewith zinc. The active site of carbonic anhydrase contains three conserved histidine residues (His94, His96 and His119).
l?uterung methode
Purify carbonic anhydrase by hydroxylapatite and DEAE-cellulose chromatography [Tiselius et al. Arch Biochem Biophys 65 132 1956, Biochim Biophys Acta 39 218 1960], and is then dialysed for crystallisation. A 0.5 to 1% solution of the enzyme in 0.05 M Tris-HCl pH 8.5 is dialysed against 1.75M solution of (NH4)2SO4 in the same buffer, and this solution is slowly increased in salt concentration by periodic removal of small amounts of dialysate and replacing with an equal volume of 3.5M (NH4)2SO4. The final salt concentration, in which the DEAE-cellulose fractions give beautiful birefringent suspensions of crystals, ranged from 2.4 to 2.7M and appeared first as fine crystals, then underwent transition to thin fragile plates. Carbonic anhydrase is a Zn enzyme which exists as several isoenzymes of varying degrees of activity [J Biol Chem 243 6474 1968, crystal structure: Nature, New Biology 235 131 1972; see also P.D. Boyer Ed. The Enzymes Academic Press NY, pp 587-665 1971].
Dehydratase, Carbonat Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
Dehydratase, Carbonat Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.
Global( 135)Lieferanten
9001-03-0(Dehydratase, Carbonat)Verwandte Suche:
Tris(pentan-2,4-dionato)eisen(II)
Tris(2,2,6,6-tetramethylheptan-3,5-dionato-O,O')dysprosium
Tris(2,2,6,6-tetramethylheptan-3,5-dionato-O,O')europium
Dichloro(ethylendiamin)platin
(Pentan-2,4-dionato-O,O')silber
1,1,3,3-Tetramethylbutylisocyanat
(Ethylenbis(nitrilomethylidyn)-2,2'-diphenolato)cobalt(II)
Phenylselenol
(p-Toluolsulfonyl)methylisocyanid
Methylisocyanacetat
N-Butylisocyanid
Ethylisocyanacetat
Kupfer(II)-4-oxopent-2-en-2-olat
Benzylisocyanid
2-Methylpropylisocyanid
Aluminiumtris(2,4-pentandionato-O,O')
- Dehydratase
- CARBONIC ANHYDRASE FROM BOVINE ERYTHRO-CYTES, >2000 U/MG*
- CARBONIC ANHYDRASE ISOZYME II, BOVINE ER YTHR., ~1800 U/MG
- CARBONIC ANHYDRASE BOVINE ERYTHROCYTESAP PROX MOL W
- CARBONIC ANHYDRASE, BOVINE ERYTHROCYTES FOR GEL FIL
- CARBONIC ANHYDRASE FROM BOVINEERYTHROCYT ES FOR SDS
- PANCREAS, BOVINE
- Dehydratase, carbonate
- TDX-02
- carbonic anhydrase from bovine erythro-cytes
- carbonic anhydrase I pi approx. 6.6
- carbonic anhydrase ii from bovine*erythrocytes pi
- carbonic anhydrase ii pi approx. 5.4
- Carbonic Anhydrase Isozyme II from bovine erythrocytes
- CARBONIC ANHYDRASE II PI APPROX. 7.4
- CARBONIC ANHYDRASE II PI APPROX. 5.9
- Carbonic Anhydrase I from human erythrocytes
- CarbonicAnhydraseExBovineErythrocytes
- Carbonic anhydrase from bovine erythrocytes ca. 1.5 U/mg
- carbonic anhydrase isozyme ii from human erythrocytes
- Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II
- Carbonate Dehydratase, Carbonate Hydrolyase
- Carbonic Anhydrase from bovine erythrocytes,Carbonate Dehydratase, Carbonate Hydrolyase
- Carbonic Anhydrase [from bovine erythrocytes, 2000 units/mg]
- Carbonic anhydrase from bovine erythrocytes, lyophil.
- Carbonic anhydrase, lyophil.
- Carbonic Anhydrase, Lyophilized Powder
- Carbonic anhydrase froM bovine erythrocytes ca. 1.5 U/Mg lyophil.
- EC 4.2.1.1
- IUB: 4.2.1.1
- CA, HUMAN LIVER
- CA1, BOVINE ERYTHROCYTES
- CARBONATE DEHYDRATASE
- CARBONATE HYDRO-LYASE
- CARBONIC ANHYDRASE BOVINE ERYTHROCYTES
- CARBONIC ANHYDRASE, HUMAN LIVER
- CARBONIC ANHYDRASE I
- CARBONIC ANHYDRASE I, BOVINE ERYTHROCYTES
- CARBONIC ANHYDRASE II
- CARBONIC ANHYDRASE ISOZYME II
- Carbonic Anhydrase Isozyme II human
- Carbonic Anhydrase from human erythrocytes
- Carbonic Anhydrase Isozyme I
- Carbonic Anhydrase II from Human, Recombinant
- Native Human Carbonic Anhydrase
- Native Human Carbonic Anhydrase I
- Native Bovine Carbonic Anhydrase
- Carbonic Anhydrase II from Bovine, Recombinant
- Carbonic Anhydrase Activity Microplate Assay Kit
- Carbonic Anhydrase I[C4396]
- Dehydratase, carbote
- CARBONIC ANHYDRASE
- 9001-03-0
- General Metabolic Enzymes
- Gel Filtration Chromatography
- General Metabolic Enzymes A-H
- Enzyme Class Index
- Enzymes, Inhibitors, and Substrates
