DiR iodide NEW
| Price | $39 | $86 | $129 |
| Package | 1mg | 5mg | 10mg |
| Supply Ability: | 10g |
| Update Time: | 2026-05-27 |
Product Details
| Product Name: DiR iodide | CAS No.: 100068-60-8 |
| Purity: 95.00% | Supply Ability: 10g |
| Release date: 2026/05/27 |
Product Introduction
Bioactivity
| Name | DiR iodide |
| Description | DiR iodide (DiR iodide [1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide]) is an important near infrared (NIR) fluorescent dye (Ex/Em=750/780 nm), which has been widely used for in vivo monitoring of cells. |
| Cell Research | Instructions: I. Solution preparation 1. Mother solution preparation: DiR iodide is prepared with DMSO at a concentration of 1-5 mM. 2. Working solution preparation: Dilute the mother solution to a suitable buffer such as serum-free culture medium, HBSS or PBS to prepare a working solution of 1 to 5 μM. II. Suspended cells 1. Centrifuge at 1000 g for 3-5 minutes at 4℃, discard the supernatant, and wash twice with PBS for 5 minutes each. The cell density is 1×10^6/mL. 2. Add 1 mL Di working solution and incubate at room temperature for 5-30 minutes. 3. Centrifuge at 400g for 3-4 minutes at 4℃ and discard the supernatant. 4. Wash twice with PBS for 5 minutes each. 5. Resuspend the cells with serum-free cell culture medium or PBS and observe with fluorescence microscopy or flow cytometry. III. Adherent cells 1. Culture adherent cells on sterile coverslips. 2. Remove the coverslip from the culture medium and aspirate the excess culture medium. 3. Add 100 μL of working solution, shake gently to completely cover the cells, and incubate at room temperature for 5-30 minutes. 4. Wash twice with culture medium, 5 minutes each time. Observe by fluorescence microscopy or flow cytometry. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| In vitro | Stain adherent cells: Grow adherent cells on sterile glass coverslips. Remove coverslips from growth medium and gently drain off excess medium. Place coverslip in a humidity chamber. Pipet 100 μL of the dye working solution onto the corner of a coverslip and gently agitate until all cells are covered. Incubate the coverslip at 37℃ for 2-20 minutes. Drain off the dye working solution and wash the coverslips two to three times with pre-warmed growth medium. Microscopy Detection: DiI, 549/565 nm, XF32-Omega, 31002-Chroma; DiR, 748/780 nm, XF112-Omega, 41009-Chroma |
| Storage | Keep away from direct sunlight,Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Keywords | DiR iodide |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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