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XBA I

XBA I Struktur
CAS-Nr.
Englisch Name:
XBA I
Synonyma:
XBA I
CBNumber:
CB5137507
Summenformel:
C97H123N38O58P9
Molgewicht:
3028
MOL-Datei:
Mol file

XBA I Eigenschaften

storage temp. 
-20°C
Aggregatzustand
solution
Biologische Quelle
Escherichia coli

Sicherheit

XBA I Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

The restriction enzyme Xba I has been used for the digestion of genomic DNA.

Allgemeine Beschreibung

Xba I recognizes the sequence T↓*CTAGA and generates fragments with 5′-cohesive termini. The enzyme needs at least two nucleotides around the target sequence before it will cut the cleavage site.

Compatible ends
Xba I ends are compatible with fragments generated by Avr I, Nhe I, and Spe I.

Isoschizomers
The enzyme is not known to have isoschizomers.

Methylation sensitivity
Xba I digestion of DNA is inhibited by the dam gene product of E. coli, which methylates the 6N position of adenine in the sequence GATC. The enzyme is also inhibited by 5-methylcytosine or 5-hydroxymethylcytosine at the site (*) indicated on the recognition sequence.

Activity in SuRE/Cut Buffer System
Buffer printed in bold face type is the buffer recommended for optimal activity:
A B L M H
100% 75-100% 75-100% 75-100% 100%

Relative activity in complete PCR mix
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 60%. The PCR mix contained DNA, primers, 10 mM Tris-HCl (pH 8.3, +20°C), 50 mM KCl, 1.5 mM MgCl2, 200 M dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles. Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 25%. When supplemented with GC-RICH Solution, activity increases to 100%.

Incubation temperature
+37°C


Number of cleavage sites on different DNAs
λ Ad2 SV40 X174 M13mp7 M13mp18 pBR322 pBR328 pUC18
1 5 0 0 0 1 0 0 1

PFGE tested
Xba I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E. coli C 600) embedded in agarose for PFGE analysis, we recommend 10 U enzyme per g DNA and approximately 4 hour incubation time.

Ligation and recutting assay
Xba I fragments obtained by complete digestion of 1 g pUC18 DNA are ligated with 1 U T4 DNA Ligase in a volume of 10 l by incubation for 16 hours at +4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C) resulting in >90% recovery of pUC18 DNA.
Subsequent re-cutting with Xba I yields >95% of the typical pattern of pUC18 × Xba I fragments.

XBA I Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


XBA I Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

Global( 8)Lieferanten
Firmenname Telefon E-Mail Land Produktkatalog Edge Rate
Nanjing Dulai Biotechnology Co., Ltd. 025-84699383-8003;025-846993838003-8003 18013301593;18013301590
njduly@126.com China 3293 55
MCE(MedChemExpress) 400-199-8808 18019480960
sales@medchemexpress.cn China 23918 58
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