ANTI-U2 AUXILIARY FACTOR Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

Monoclonal Anti-U2AF⁶⁵ antibody produced in mouse has been used in:

• RNA immunoprecipitation
• immunoblotting
• chromatin immunoprecipitation (ChIP)
• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry
• immunocytochemistry
• immunoelectron microscopy

Allgemeine Beschreibung

Monoclonal Anti-U2AF⁶⁵ (mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF⁶⁵. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF⁶⁵) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF⁶⁵kDa subunit is a non-snRNP protein component of spliceosome complexes.

Biochem/physiol Actions

U2 snRNP auxiliary factor 65 kDa (U2AF65)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF65 primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF65) and (U2AF35) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus.

ANTI-U2 AUXILIARY FACTOR Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

ANTI-U2 AUXILIARY FACTOR Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51395	80

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• Monoclonal Anti-U2AF65 antibody produced in mouse
• ANTI-U2AF65
• ANTI-U2 AUXILIARY FACTOR
• MONOCLONAL ANTI-U2AF65
• Primary Antibodies
• Antibodies for Gene Regulation and Expression
• Antibodies for RNA Regulation and Translation
• Antibodies
• Antibodies to Splicing Factors
• Cell Biology
• BioChemical
• Antibodies to Splicing FactorsCell Biology
• Alphabetical Index
• Antibodies for Gene Regulation and Expression
• Antibodies for RNA Regulation and Translation
• Antibodies to Transcription RegulatorsAntibodies
• Clone Index
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