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ChemicalBook >> CAS DataBase List >>Hematoxylin

Hematoxylin

CAS No.
517-28-2
Chemical Name:
Hematoxylin
Synonyms
Homaeoxylin;hematoxyline;hydroxybrazilin;Hematoxylin powder;HEMATOXYLIN CERTIFIED;HEMATOXYLIN INDICATOR;C.I.75290;nci-c55889;Nsc 270085;Color fine
CBNumber:
CB1382426
Molecular Formula:
C16H14O6
Molecular Weight:
302.28
MDL Number:
MFCD00283323
MOL File:
517-28-2.mol
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-07-01 15:51:14
Product description Number Pack Size Price
Hematoxylin cryst. (C.I. 75290) for microscopy 1.04302 25g $228
Hematoxylin cryst. (C.I. 75290) for microscopy 1.04302 100g $648
Hematoxylin cryst. (C.I. 75290) for microscopy 1.04302 1kg $7200
Hematoxylin HARLECO? Nuclear staining 234 25g $122
Hematoxylin HARLECO? Nuclear staining 234 100g $424
More product size

Hematoxylin Properties

Melting point 200 °C (dec.)(lit.)
Boiling point 363.32°C (rough estimate)
Density 1.2514 (rough estimate)
bulk density 500kg/m3
vapor pressure 0-0Pa at 20-25℃
refractive index 1.4600 (estimate)
storage temp. Keep in dark place,Inert atmosphere,Room temperature
solubility Soluble in 95% ethanol(1 mg/mL).
form Powder/Solid
Colour Index 75290
pka 6.7(at 25℃)
color Yellow to Brown
PH Range Red (0.0) to yellow (1.0);Pale yellow (5.0) to violet (6.0)
optical activity 77.5°(C=0.01g/mL, MEOH, 20°C, 589nm)
Water Solubility SOLUBLE IN HOT WATER
Sensitive Light Sensitive
λmax 292nm, 445nm, 560nm
Merck 14,4637
BRN 91399
Stability Stable, but may discolour on exposure to light. Incompatible with strong oxidizing agents.
Major Application Plasma displays, textiles, hair dyes, identifying fresh and stale rice, diag-nosing cancer progression, detecting nosing cancer progression, cervical disease, central nervous system malfunctions, detecting genes, breast cancer, collagen in a tissue sample, apoptosis, demyelinating diseases, antigens, treatment of age-related macular degeneration, burns, prostate cancer, diabetesand obesity, viral diseases, neoplasms, peripheral neural and vascular ailments, skin disorders, biotechnological applications, reference standard materials for cytology, histology andimmunohistochemistry
InChI 1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1
InChIKey WZUVPPKBWHMQCE-UHFFFAOYSA-N
SMILES Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O
LogP 0.3 at 30℃ and pH6.9
CAS DataBase Reference 517-28-2
EWG's Food Scores 1
FDA UNII YKM8PY2Z55
EPA Substance Registry System Hematoxylin (517-28-2)
UNSPSC Code 41116130
NACRES NA.47

SAFETY

Risk and Safety Statements

Symbol(GHS)  Exclamation Mark (GHS07)
GHS07
Signal word  Warning
Hazard statements  H319
Precautionary statements  P264-P280-P305+P351+P338-P337+P313
PPE dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes  Xn,Xi
Risk Statements  22-36/37/38
Safety Statements  26-36
WGK Germany  2
RTECS  MH7875000
TSCA  TSCA listed
HS Code  32030019
Storage Class 11 - Combustible Solids
Hazard Classifications Eye Irrit. 2
Hazardous Substances Data 517-28-2(Hazardous Substances Data)
REACH Registrations Active
NFPA 704
1
2 0

Hematoxylin price More Price(104)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 1.04302 Hematoxylin cryst. (C.I. 75290) for microscopy 517-28-2 25g $228 2026-04-30 Buy
Sigma-Aldrich 1.04302 Hematoxylin cryst. (C.I. 75290) for microscopy 517-28-2 100g $648 2026-04-30 Buy
Sigma-Aldrich 1.04302 Hematoxylin cryst. (C.I. 75290) for microscopy 517-28-2 1kg $7200 2026-04-30 Buy
Sigma-Aldrich 234 Hematoxylin HARLECO? Nuclear staining 517-28-2 25g $122 2023-01-07 Buy
Sigma-Aldrich 234 Hematoxylin HARLECO? Nuclear staining 517-28-2 100g $424 2023-01-07 Buy
Product number Packaging Price Buy
1.04302 25g $228 Buy
1.04302 100g $648 Buy
1.04302 1kg $7200 Buy
234 25g $122 Buy
234 100g $424 Buy

Hematoxylin Chemical Properties,Uses,Production

Chemical Properties

light brown crystals or powder

Uses

Hematoxylin and eosin together make up Hematoxylin and eosin stain, one of the most commonly used stains in histology. Metal-haematein complexes are used to stain cell nuclei prior to examination under a microscope. Structures that stain with iron- or aluminum-haematein are often called basophilic, even though the mechanism of the staining is different from that of staining with basic dyes.

Uses

Colorant in inks, biological stains.

Definition

ChEBI: (+)-haematoxylin is a haematoxylin. It is an enantiomer of a (-)-haematoxylin.

General Description

White to yellowish crystals that redden on exposure to light.

Air & Water Reactions

Soluble in hot water, slightly water soluble in cold .

Reactivity Profile

Sensitive to light. .

Hazard

May be carcinogenic.

Biological Activity

Hematoxylin is an organic stain which chelates with metal ions and binds to DNA and RNA. It is used for staining nucleus of the cells. Hematoxylin along with eosin stain is used in the diagnosis of invasion stage in tumors.

storage

Store at RT

Purification Methods

Hematoxylin recrystallises from H2O (as trihydrate) in white-yellow crystals which become red on exposure to light and then melt at 100-120o. It has been recrystallised from Me2CO/*C6H6. It has been recrystallised as well from dilute aqueous NaHSO3 until colourless and is soluble in alkali, borax and glycerol. Store it in the dark below 0o. [Morsingh & Robinson Tetrahedron 26 182 1970, Dann & Hofmann Chem Ber 98 1498 1955, Beilstein 17/8 V 469.]

Description

Hematoxylin is a blue nuclear counterstain for use in immunohistochemical assays. It yields crisp staining detail with superior contrast when used in conjunction with SignalStain? DAB Substrate Kit

Hematoxylin Preparation Products And Raw materials

Raw materials

1of2

Preparation Products

Global( 572)Suppliers
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Hebei Chuanghai Biotechnology Co., Ltd
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Related Qustion

Ask a question
Q:How to prepare hematoxylin solution for acid-base indicating?Walter - Jul 1,2026
A:When preparing Hematoxylin solution, add 0.5 g in 100 mL water. It acts as pH indicator. Between pH 5.0-7.2, its color transitions gradually from yellow in acidic conditions to violet in basic conditions.

View Lastest Price from Hematoxylin manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Hematoxylin pictures 2026-08-01 Hematoxylin
517-28-2
1KG >99% HPLC 1000KG Changsha Staherb Natural Ingredients Co., Ltd.
Hematoxylin pictures 2026-08-01 Hematoxylin
517-28-2
0.99 RongNa Biotechnology Co.,Ltd
Hematoxylin pictures 2026-08-01 Hematoxylin
517-28-2
1KG 98%min 30tons/month WUHAN FORTUNA CHEMICAL CO., LTD
  • Hematoxylin pictures
  • Hematoxylin
    517-28-2
  • $0.00
  • >99% HPLC
  • Changsha Staherb Natural Ingredients Co., Ltd.
  • Hematoxylin pictures
  • Hematoxylin
    517-28-2
  • $0.00
  • 98%min
  • WUHAN FORTUNA CHEMICAL CO., LTD
GILL 2 METHOD HEMATOXYLIN STAIN GILL HEMATOXYLIN SOLUTION NO II GILL'S HEMATOXYLIN NO 2 GILL'S HEMATOXYLIN SOLUTION NO 2 GILLS II HAEMATOXYLIN HEMATOXYLIN STAIN, GILL 2 HEMATOXYLIN SOLUTION GILL NO 2 HEMATOXYLIN, GILL II HEMATOXYLIN, GILL'S NO 2 2-d)pyran-3,4,6a,9,10(6h)-pentol,7,11b-dihydro-benz(b)indeno( 2-d]pyran-3,4,6a,9,10(6H)-pentol,7,11b-dihydro-,cis-(+)-Benz[b]indeno[1 4,6a,9,10(6h)-pentol,7,11b-dihydro-2-d)pyran-(6as-cis)-benz(b)indeno( 4,6a,9,10(6h)-pentol,7,11b-dihydro-2-d)pyran-cis-(+)-benz(b)indeno( 4,6a,9,10(6h)-pentol,7,11b-dihydro-2-d]pyran-cis-(++)-benz[b]indeno[ abcolhematoxylin-certified nci-c55889 bematoxylin h(a)ematine HEMATOXYLIN FOR MICROSCOPY HEMATOXYLIN, CERTIFIED (C.I. 75290) HEMATOXYLIN C.I. 75290 BIOLOGICAL STAIN HEMATOXYLIN C.I. 75290 CERTIFIED (NATURAL BLACK 1) Hematoxylin, certified, pure, 90% Hematoxylin, pure, 85% Benzbindeno1,2-dpyran-3,4,6a,9,10(6H)-pentol, 7,11b-dihydro-, (6aS,11bR)- Haematoxylin(Harris)Solution HematoxylinStain`Certified` Haematoxylin(Ehrlich)AcidSolution Haematoxalin(Heidenhain'SNo1-Iron) Haematoxylin(Delafield)Solution Haematoxylin(WeigertB-Iron)Solution Haematoxylin(Heidenhain'SNo2)Solution HematoxylinStainCertified-C.I.75290 HaematoxylinPhosphotungstic(Mallory) HEMATOXYLIN STAIN C.I.No 75290 [7,11b]-Dihydrobenz [b] indeno [1,2-d] pyran-3,4,6α,9,10-[6H]-pentol Hematoxylin hydrate, high purity biological stain, pure Hematoxylin 7,11b-Dihydrobenz[b]indeno[1,2-d]pyran-3,4,6a,9,10(6H)-pentol CAP DIN 45 FOR GLASS BOTTLE NO PTFE CAP DIN 45 FOR GLASS BOTTLE WITH PTFE CAP PE BLACK DIN 61 Hematoxylin, 96% dry weight Hematoxylin for Biological Stain Nsc 270085 Benz[b]indeno[1,2-d]pyran-3,4,6a,9,10(6h)-pentol, 7,11b-dihydro, (6as-cis)- Hematoxylin cryst. (C.I. 75290) for microscopy Hematoxylin, H & E Stain Hematoxylin, H & E Stain, For Microscopy HAEMATOXYLIN(RG) HEMATOXYLIN HYDRATE (DRY WT. BASIS), WATER CA 6% 7,11b-Dihydrobenz[b]indeno[1,2-d]pyran-3,4,6a,9,10(6H)-pentol C.I.75290 Hematoxylin Natural Black 1 HAEMATOXYLIN (C.I. 75290) CI 75290, Hydroxybraslin, Natural Black 1 Hematoxylin hydrate, 96% (dry wt.), water ca 6% Hematoxylin hydrate, 96% (dry wt. basis), water ca 6%
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