Cellular Glutathione Detection Assay Kit
- CAS No.
- Chemical Name:
- Cellular Glutathione Detection Assay Kit
- Synonyms
- Cellular Glutathione Detection Assay Kit
- CBNumber:
- CB25724334
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
Cellular Glutathione Detection Assay Kit Chemical Properties,Uses,Production
Description
The Cellular Glutathione Detection Assay Kit employs the cell permeable dye monochlorobimane (MCB) to detect reduced glutathione (GSH) in cellular assays. MCB displays a high affinity for reduced glutathione and exhibits a very low fluorescent yield when free in solution. Upon binding to GSH, the dye exhibits a strong blue fluorescence that can be measured at an excitation wavelength of 380 nm and an emission wavelength of 460 nm. Fluorescent intensity correlates with sample GSH level. This kit can be used to either label cells directly or to detect GSH level in cell extracts. The assay can be easily applied in high throughput plate-format, flow cytometry, or fluorescent imaging.
Reactivity
All
Background
The antioxidant glutathione is found in both reduced and oxidized states in cells. Reduced glutathione can play an important role in preventing cellular damage caused by reactive oxygen species, including free radicals and peroxides. Reduced glutathione acts as an electron donor in the presence of free radicals and peroxides to become oxidized. GSH also participates in redox signaling through the removal of the cellular second messenger H2O2. Diminished glutathione levels are observed during the aging process and in oxidative stress-related diseases. The depletion of GSH is necessary for the progression of apoptosis that is mediated by various signaling pathways. Intracellular GSH levels can be a very useful indicator for overall cell health, proliferation, and death.
References
[1] Dickinson, D.A. and Forman, H.J. (2002) Ann N Y Acad Sci 973, 488-504.
[2] Pompella, A. et al. (2003) Biochem Pharmacol 66, 1499-503.
[3] Franco, R. et al. (2007) J Biol Chem 282, 30452-65.
[4] Macho, A. et al. (1997) J Immunol 158, 4612-9.
Cellular Glutathione Detection Assay Kit Preparation Products And Raw materials
Raw materials
Preparation Products
Cellular Glutathione Detection Assay Kit Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Shanghai Universal Biotech Co.,Ltd | 15921930842 15921930842 | yh-wang@univ-bio.com | China | 25018 | 58 |
| Cell Signaling Technology Inc | -- | info@cellsignal.cn | China | 6725 | 58 |
| Supplier | Advantage |
|---|---|
| Shanghai Universal Biotech Co.,Ltd | 58 |
| Cell Signaling Technology Inc | 58 |
