Description

Monoclonal antibodies are produced by immunizing mouse with full length His-tagged p53 protein.

Source

Rabbit

Applications

Western Blot, Immunoprecipitation.

Reactivity

Human;Monkey

Background

p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein p53 senses DNA damage and possibly facilitating repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

References

[1] Levine, A.J. (1997) Cell 88, 323-31.
[2] Meek, D.W. (1994) Semin Cancer Biol 5, 203-10.
[3] Milczarek, G.J. et al. (1997) Life Sci 60, 1-11.
[4] Shieh, S.Y. et al. (1997) Cell 91, 325-34.
[5] Chehab, N.H. et al. (1999) Proc Natl Acad Sci U S A 96, 13777-82.
[6] Honda, R. et al. (1997) FEBS Lett 420, 25-7.
[7] Tibbetts, R.S. et al. (1999) Genes Dev 13, 152-7.
[8] Shieh, S.Y. et al. (1999) EMBO J 18, 1815-23.
[9] Hirao, A. et al. (2000) Science 287, 1824-7.
[10] Hao, M. et al. (1996) J Biol Chem 271, 29380-5.

Raw materials

Preparation Products