Source

Rabbit

Applications

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Recommended starting dilution is 1:1000.

Reactivity

Human;Monkey

Immunogen

Anti-human ASF1B mAb, clone PAT1D4AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ASF1B protein (1-202 amino acids) purified from E. coli.

Background

ASF1 Anti-Silencing Function 1 Homolog B (ASF1B) belongs to the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 protein in yeast. ASF1B is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may have a crucial role in modulating the nucleosome structure of chromatin by guaranteeing a regular supply of histones at sites of nucleosome assembly. ASF1B cooperates with CAF-1 (chromatin assembly factor 1) to stimulate replication-dependent chromatin assembly. ASF1B is highly expressed in the testis and at lower levels in colon, small intestine and thymus. ASF1B is necessary for spermatogenesis.

References

[1] Singer, M.S. et al. (1998) Genetics 150, 613-632.
[2] Mousson, F. et al. (2007) Chromosoma 116, 79-93.
[3] Tang, Y. et al. (2006) Nat. Struct. Mol. Biol. 13, 921-929.
[4] Zhang, R. et al. (2005) Dev. Cell. 8, 19-30.
[5] Daganzo, S.M. et al. (2003) Curr. Biol. 13, 2148-2158.
[6] Ramey, C.J. et al. (2004) Mol. Cell. Biol. 24, 10313-10327.
[7] Prado, F. et al. (2004) EMBO Rep. 5, 497-502.
[8] Tyler, J.K. et al. (1999) Nature 402, 555-560.
[9] Sanematsu, F. et al. (2006) J. Biol. Chem. 281, 13817-13827.
[10] Groth, A. et al. (2005) Mol. Cell. 17, 301-311.

Raw materials

Preparation Products