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Phosphatase Inhibitor Cocktail (100X)

CAS No.
Chemical Name:
Phosphatase Inhibitor Cocktail (100X)
Synonyms
Phosphatase Inhibitor Cocktail (100X)
CBNumber:
CB56264342
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file

Phosphatase Inhibitor Cocktail (100X) Chemical Properties,Uses,Production

Description

This Phosphatase Inhibitor Cocktail is used to prevent dephosphorylation of phosphorylated proteins from active serine/threonine and tyrosine phosphatases present in whole cell extract. The 100X cocktail is a colorless to light yellow liquid.

Background

Dynamic protein phosphorylation is a key cellular signaling mechanism by which a broad spectrum of cellular processes is regulated. In order to study the phosphorylation status of specific target proteins the phosphorylated residue of interest must remain intact. When cells are lysed to make whole cell extracts, a loss of normal cellular signaling regulation occurs, and phosphatases within the cell extract are free to dephosphorylate proteins in an uncontrolled manner. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption.

Phosphatase Inhibitor Cocktail (100X) Preparation Products And Raw materials

Raw materials

Preparation Products

Phosphatase Inhibitor Cocktail (100X)
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