Source

Mouse

Reactivity

Human;Monkey;Bovine;Pig

Background

The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

References

[1] Sherr, C.J. (1996) Science 274, 1672-7.
[2] Nevins, J.R. (1992) Science 258, 424-9.
[3] Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
[4] Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
[5] Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
[6] Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
[7] Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
[8] Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
[9] Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

Raw materials

Preparation Products