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ChemicalBook >> CAS DataBase List >>Neutral Red

Neutral Red

CAS No.
553-24-2
Chemical Name:
Neutral Red
Synonyms
Neutral Red;Basic Red 5;N2,N2,7-TriMethylphenazine-2,8-diaMine hydrochloride;Kernechrot;C.I. 50040;neutralredw;c.i.basicred5;michromeno.226;cervenneutralni;cervenzasadita5
CBNumber:
CB7853810
Molecular Formula:
C15H16N4.ClH
Molecular Weight:
288.78
MDL Number:
MFCD00012651
MOL File:
553-24-2.mol
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-07-16 07:06:08
Product description Number Pack Size Price
Neutral Red for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0) 72210 5g $35.3
Neutral red (C.I. 50040) indicator and for microbiology 1.01369 25g $224
Neutral red (C.I. 50040) indicator and for microbiology 1.01369 100g $785
Neutral red (C.I. 50040) for microscopy Certistain? 1.01376 25g $149
Basic Red 5 N0315 25g $50
More product size

Neutral Red Properties

Melting point 290 °C (dec.) (lit.)
Boiling point 441.31°C (rough estimate)
Density 1.1590 (rough estimate)
bulk density 350-500kg/m3
refractive index 1.6110 (estimate)
storage temp. Store at +5°C to +30°C.
solubility H2O: soluble10mg/mL
form Solid
Colour Index 50040
pka 6.7, 7.4(at 25℃)
color Very dark green
PH Range 6.8(red)-8(yellow)
PH 3.1 (10g/l, H2O, 20℃)
Odor Odorless
Appearance Solid Powder
Water Solubility 50 g/L
λmax 540nm, 533nm, 544nm, 529nm, 454nm
Merck 14,6488
BRN 3918740
Stability Stable. Incompatible with strong oxidizing agents.
Biological Applications Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases
Major Application Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases
InChI 1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
InChIKey PGSADBUBUOPOJS-UHFFFAOYSA-N
SMILES Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
CAS DataBase Reference 553-24-2(CAS DataBase Reference)
FDA UNII 261QK3SSBH
EPA Substance Registry System Neutral Red (553-24-2)
UNSPSC Code 41116130
NACRES NB.21

SAFETY

Risk and Safety Statements

Symbol(GHS)  Exclamation Mark (GHS07)
GHS07
Signal word  Warning
Hazard statements  H315-H319-H335
Precautionary statements  P280-P302+P352
PPE Eyeshields, Gloves, type N95 (US)
Hazard Codes  Xn,Xi
Risk Statements  68-36/37/38
Safety Statements  36/37-37/39-36-26-24/25
WGK Germany  3
RTECS  SG1400000
TSCA  TSCA listed
HS Code  32041300
Storage Class 11 - Combustible Solids
Toxicity LD50 intraperitoneal in mouse: 432mg/kg

Neutral Red price More Price(109)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 72210 Neutral Red for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0) 553-24-2 5g $35.3 2026-04-30 Buy
Sigma-Aldrich 1.01369 Neutral red (C.I. 50040) indicator and for microbiology 553-24-2 25g $224 2026-04-30 Buy
Sigma-Aldrich 1.01369 Neutral red (C.I. 50040) indicator and for microbiology 553-24-2 100g $785 2026-04-30 Buy
Sigma-Aldrich 1.01376 Neutral red (C.I. 50040) for microscopy Certistain? 553-24-2 25g $149 2023-01-07 Buy
TCI Chemical N0315 Basic Red 5 553-24-2 25g $50 2026-04-30 Buy
Product number Packaging Price Buy
72210 5g $35.3 Buy
1.01369 25g $224 Buy
1.01369 100g $785 Buy
1.01376 25g $149 Buy
N0315 25g $50 Buy

Neutral Red Chemical Properties,Uses,Production

Description

Neutral red is useful as a biological stain and as an indicator for preparing neutral red paper.

Chemical Properties

dark green powder

Uses

Can be used as a vital stain, to stain living cells.

Uses

Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.

Preparation

(a) N,N-dimethyl-4-nitrosobenzenamineand 4-Methylbenzene-1,3-diaminereaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine, and 4-Methylbenzene-1,3-diamine mixture.

Application

Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.

Definition

ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).

General Description

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Biochem/physiol Actions

Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Biotechnological Applications

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

storage

+4°C

Staining Procedures

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

Properties and Applications

red light purple and red. Soluble in water is cherry red, soluble in ethanol for product red colour with red fluorescent light brown. The strong sulfuric acid for green, blue for diluted, and then into a product red. The dye solution to join strong sodium hydroxide solution have yellow brown precipitation.

Purification Methods

In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]

Neutral Red Preparation Products And Raw materials

Global( 434)Suppliers
Supplier Tel Email Country ProdList Advantage
RongNa Biotechnology Co.,Ltd
+86-86-13583358881 +8618560316533 Brad@rongnabiotech.com China 3375 58
Henan Tianfu Chemical Co.,Ltd.
+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21585 55
career henan chemical co
+86-0371-86658258 sales@coreychem.com China 29812 58
SHANDONG ZHI SHANG CHEMICAL CO.LTD
0531-68652808 18953170293 sales@sdzschem.com China 2921 58
Shandong chuangyingchemical Co., Ltd.
18853181302 sale@chuangyingchem.com CHINA 5906 58
BOC Sciences
+1-631-485-4226 inquiry@bocsci.com United States 19936 58
Shenyang Simchoice Chemical Co.,Ltd
+86-024-23769576 +86-15040101888 sales@simchoicechem.com China 254 58
CONIER CHEM AND PHARMA LIMITED
sales@conier.com China 49979 58
TargetMol Chemicals Inc.
+1-781-999-5354; +1-00000000000 marketing@targetmol.com United States 32468 58
Hefei TNJ Chemical Industry Co.,Ltd.
+86-0551-65418671 +86-189 4982 3763 sales@tnjchem.com China 34563 58

Related Qustion

Ask a question
Q:How to prepare a Neutral Red (C.I. 50040) indicator solution?STAR - Jul 2,2026
A:0.1 / 0.3 g of Neutral Red in 100 mL of ethanol (70%) or 0.2 g of Neutral Red in 100 mL of methanol.

View Lastest Price from Neutral Red manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Neutral Red pictures 2026-08-04 Neutral Red
553-24-2
$10.00 1ASSAYS 99% 1 ton RongNa Biotechnology Co.,Ltd
Basic Red 5 pictures 2026-08-04 Basic Red 5
553-24-2
1KG 98%min 30tons/month WUHAN FORTUNA CHEMICAL CO., LTD
Neutral red pictures 2026-07-15 Neutral red
553-24-2
$41.00 99.60% 10g TargetMol Chemicals Inc.
  • Neutral Red pictures
  • Neutral Red
    553-24-2
  • $10.00
  • 99%
  • RongNa Biotechnology Co.,Ltd
  • Basic Red 5 pictures
  • Basic Red 5
    553-24-2
  • $0.00
  • 98%min
  • WUHAN FORTUNA CHEMICAL CO., LTD
  • Neutral red pictures
  • Neutral red
    553-24-2
  • $41.00
  • 99.60%
  • TargetMol Chemicals Inc.
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