NAI-N3
- CAS No.
- 1612756-29-2
- Chemical Name:
- NAI-N3
- Synonyms
- NAI 1;NAI-N3;2-(Azidomethyl)nicotinic acid imidazolide;[2-(Azidomethyl)-3-pyridinyl]-1H-imidazol-1-yl-methanone;Methanone, [2-(azidomethyl)-3-pyridinyl]-1H-imidazol-1-yl-
- CBNumber:
- CB83356166
- Molecular Formula:
- C10H8N6O
- Molecular Weight:
- 228.21
- MDL Number:
- MFCD31560475
- MOL File:
- 1612756-29-2.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Product description | Number | Pack Size | Price |
| 2-(Azidomethyl)nicotinic acid imidazolide ≥95% | 913812 | 50mg | $193 |
| 2-(Azidomethyl)nicotinic acid imidazolide ≥95% | 913812 | 250 mg | $742 |
| 2-(Azidomethyl)nicotinic acid imidazolide ≥95% | 913812 | 250 mg | $742 |
| NAI 1 ≥98%(HPLC) | 460491 | 5mg | $355 |
| NAI 1 ≥98%(HPLC) | 460491 | 10mg | $496 |
| Melting point | 74-80°C |
|---|---|
| storage temp. | Store at -20°C |
| solubility |
Ethanol:16.67(Max Conc. mg/mL);73.05(Max Conc. mM) DMSO:250.0(Max Conc. mg/mL);1095.46(Max Conc. mM) |
| form | Solid |
| pka | 2.364±0.10(predicted) |
| color | White to Pale Beige |
| Stability | Light sensitive, Moisture sensitive |
| InChI | 1S/C10H8N6O/c11-15-14-6-9-8(2-1-3-13-9)10(17)16-5-4-12-7-16/h1-5,7H,6H2 |
| InChIKey | QPSQVTFTPHUCEH-UHFFFAOYSA-N |
| SMILES | [N+](=[N-])=NCc1ncccc1C(=O)[n]2cncc2 |
| UNSPSC Code | 12352106 |
| NACRES | NA.21 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS08,GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H373-H312-H319-H315-H332-H335-H302 |
| Precautionary statements | P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P-P261-P271-P304+P340-P312-P260-P314-P501-P264-P270-P301+P312-P330-P501-P280-P302+P352-P312-P322-P363-P501 |
| WGK Germany | WGK 3 |
| Storage Class | 5.2 - Organic peroxides and self-reacting hazardous materials |
| Hazard Classifications | Eye Irrit. 2 Self-react. C Skin Irrit. 2 |
NAI-N3 price
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 913812 | 2-(Azidomethyl)nicotinic acid imidazolide ≥95% | 1612756-29-2 | 50mg | $193 | 2025-07-31 | Buy |
| Sigma-Aldrich | 913812 | 2-(Azidomethyl)nicotinic acid imidazolide ≥95% | 1612756-29-2 | 250 mg | $742 | 2025-07-31 | Buy |
| Sigma-Aldrich | 913812 | 2-(Azidomethyl)nicotinic acid imidazolide ≥95% | 1612756-29-2 | 250 mg | $742 | 2025-07-31 | Buy |
| Usbiological | 460491 | NAI 1 ≥98%(HPLC) | 1612756-29-2 | 5mg | $355 | 2026-06-03 | Buy |
| Usbiological | 460491 | NAI 1 ≥98%(HPLC) | 1612756-29-2 | 10mg | $496 | 2026-06-03 | Buy |
NAI-N3 Chemical Properties,Uses,Production
Description
NAI-N3 is a probe for icSHAPE (in vivo click selective 2'-hydroxyl acylation and profiling experiment). It acylates the 2'-OH in ssRNA and enables RNA structure profiling in vivo and ex vivo for all four bases. NAI-N3 can be used for site-specific RNA acylation using the RAIL (RNA Acylation at Induced Loops) method or in Apta-Seq to discover new aptamers in selected pools.
Uses
NAI 1 is used in the process of visualizing the physical basis for molecular behaviours inside living cells. RNAs are central to biological regulation and this molecule aids in the first global view of RNA secondary structures in living cells for all four bases.
in vitro
In brief, the bifunctional NAI-N3 SHAPE reagent is used to label all RNAs inside living cells (or in vitro refolded RNA) to generate covalent modifications on targeted RNAs at single-nucleotide resolution. _x000D_ _x000D_ Reference: Nat Protoc. 2016 Feb;11(2):273-90. https://pubmed.ncbi.nlm.nih.gov/26766114/
in vivo
This study has tested the NAI-N3 reagent in bacterial (Gram-negative Escherichia coli), yeast, insect and mammalian cells for SHAPE experiments. All of these systems proved to be amenable for icSHAPE modification and RNA structure determination. This study has successfully generated icSHAPE data from experiments in which the in vivo modification occurs at temperatures between 30 and 37 °C, to accommodate the specific culture temperatures. Thus, the procedure can be easily adapted to a wide variety of culture systems to allow transcriptome-wide RNA structure determination in most model systems._x000D_ _x000D_ Reference: Nat Protoc. 2016 Feb;11(2):273-90. https://pubmed.ncbi.nlm.nih.gov/26766114/
target
NAI-N3 is a RNA acylation reagent that enables RNA purification. NAI-N3 is a dual-function SHAPE (selective 2-hydroxyl acylation and profiling experiment) probe (RNA structure probe and enrichment).
NAI-N3 Preparation Products And Raw materials
Raw materials
Preparation Products
NAI-N3 Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| TargetMol Chemicals Inc. | +1-781-999-5354; +17819995354 | marketing@targetmol.com | United States | 32466 | 58 |
| Shenyang Zhongshen Zekang Biomedical Technology Research Co., Ltd | +86-18341751992 +86-15382112998 | 757984502@qq.com | China | 892 | 58 |
| Jilin Chinese Academy of Sciences-yanshen Technology | +undefined18143011203 | info@chemextension.com | China | 42056 | 58 |
| Xian confluore Biological Technology Co., Ltd. | +86-15680926068 | 1924344760@confluore.cn | China | 2997 | 58 |
| Aladdin Scientific | tp@aladdinsci.com | United States | 52923 | 58 | |
| BOC Sciences | 1-631-485-4226; 16314854226 | info@bocsci.com | United States | 9920 | 65 |
| MedChemexpress LLC | 021-58955995 | sales@medchemexpress.cn | United States | 4861 | 58 |
| Shanghai Macklin Biochemical Co.,Ltd. | 15221275939; 15221275939 | shenlinxing@macklin.cn | China | 15781 | 55 |
| Shanghai EFE Biological Technology Co., Ltd. | 021-65675885 18964387627 | info@efebio.com | China | 9804 | 58 |
| Xi'an Confluore Biological Technology Co., Ltd. | +86-156-80926068 +86-15680926068 | 1924344760@qq.com | China | 4953 | 58 |
