Source

Rabbit

Reactivity

Human

Background

LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.

References

[1] Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83.
[2] Zhang, W. et al. (1998) Cell 92, 83-92.
[3] Paz, P. E. et al. (2001) Biochem. J. 356, 461-71.
[4] Zhang, W. et al. (2000) J. Biol. Chem. 275, 23355-61.

Raw materials

Preparation Products