CK 666;CK-0944666;CK-666 ，S7690;CK 666
(CK 0944666);CK-666, 10 mM in DMSO;Arp2/3 Complex Inhibitor I, CK-666;CK 666, Actin polymerization inhibitor;2-Fluoro-N-[2-(2-methyl-3-indolyl)ethyl]benzamide;2-Fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide;Benzamide, 2-fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]-
CK-666 may be used to study Arp2/3-mediated structural changes in cells.
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A cell-permeable indolyl-fluorobenzamide compound that selectively inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human, bovine, fission yeast S. pombe, and budding yeast S. cerevisiae origin (IC50 = 4, 17, 5, and 12 μM, respectively), without affecting S. pombe formin domain Cdc12(FH2)-mediated or the spontaneous actin polymerization. Shown to inhibit the actin filament "comet tails" formation around intracellular Listeria in infected SKOV3 cells (IC50 = 7 μM) in a reversible manner. Structrual studies indicate that CK-666 targets a pocket formed between subdomain 4 of Arp2 and subdomain 1 of Arp3, preventing Arp2/3 from shifting into an active conformation upon N-WASP-VCA binding. CK-666 and CK-869 (Cat. No. 182516) exhibit different modes of binding, resulting in their different yeast cross-reactivities. CK-689 (Cat. No. 182517) can serve as a negative control.
Biochem/physiol Actions
CK-666 binds to Arp2/3 complex, stabilizes the inactive state of the complex and prevents its movement into active conformation.
