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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
imp.E imp.E 950781-89-2 C13H12F3N3O3
Lidocaine EP Impurity Lidocaine EP Impurity 31785-64-5 C12H18N2O.ClH
Prucalopride Impurity 1 Prucalopride Impurity 1 1900715-96-9 C18H24ClN3O3
3-(5-CHLOROPYRID-2-YL)CARBAMOYLPYRAZINE-2-CARBOXYLIC ACID(WXG01564) 3-(5-CHLOROPYRID-2-YL)CARBAMOYLPYRAZINE-2-CARBOXYLIC ACID(WXG01564) 43200-83-5 C11H7ClN4O3
Ampicillin Impurity Q Ampicillin Impurity Q
Cefdinir Impurity  PQ(CP) Cefdinir Impurity PQ(CP)
Cefdinir Impurity IJKL Cefdinir Impurity IJKL
Ceftazidime Impurity A (EP) Ceftazidime Impurity A (EP)
Oxacillin Sodium Impurity E(EP) Oxacillin Sodium Impurity E(EP)
Spectinomycin Impurity B Spectinomycin Impurity B
Eriodictyol-8-glucoside Eriodictyol-8-glucoside 153733-96-1 C21H22O11
Naringin 4'-glucoside Naringin 4'-glucoside 17257-21-5 C33H42O19
8-(benzo[b]thiophen-4-yl)-5,8-diazaspiro[4.5]decan-5-ium chloride 8-(benzo[b]thiophen-4-yl)-5,8-diazaspiro[4.5]decan-5-ium chloride 2143944-64-1 C16H21N2S+
2-(4'-chloro-2,3,4,5-tetrahydro-[1,1'-biphenyl]-4-yl)-3-hydroxynaphthalene-1,4-dione 2-(4'-chloro-2,3,4,5-tetrahydro-[1,1'-biphenyl]-4-yl)-3-hydroxynaphthalene-1,4-dione 1809464-27-4 C22H17ClO3
Aprepitant impurity C Aprepitant impurity C
Ticagrelor Related Compound 14 Ticagrelor Related Compound 14 2056269-94-2 C23H28F2N6O4S
Cefoxitin impurity E Cefoxitin impurity E 2395025-32-6 C17H19N3O8S2
(E)-2-(6-(methoxycarbonyl)-2-oxoindolin-3-ylidene)-2-phenylacetic acid (E)-2-(6-(methoxycarbonyl)-2-oxoindolin-3-ylidene)-2-phenylacetic acid C18H13NO5
8-Epi-cyanocobalamin 8-Epi-cyanocobalamin 41325-63-7 C63H88CoN14O14P
Lamivudine Impurity 2 Lamivudine Impurity 2 200396-21-0 C18H27N3O4S
Lamivudine Impurity 1 Lamivudine Impurity 1 1235712-40-9 C8H11N3O4S
Moxifloxacin Related Impurity 3 Moxifloxacin Related Impurity 3
Bisoprolol EP Impurity Q Bisoprolol EP Impurity Q 1346604-00-9 C16H27NO4
Levofloxacin N-Oxide Levofloxacin N-Oxide 117678-37-2 C18H20FN3O5
Oseltamivir EP Impurity A Oseltamivir EP Impurity A 1391047-93-0 C14H24N2O4
Quetiapine EP Impurity N Quetiapine EP Impurity N 1800291-86-4 C29H41N5O3S
Ceftazidime Impurity Ceftazidime Impurity
Chlorhexidine Digluconate Impurity N Chlorhexidine Digluconate Impurity N 152504-10-4 C15H25ClN8
S-Azetidin-3-yl ethanethioate HCl S-Azetidin-3-yl ethanethioate HCl 886615-18-5 C5H9NOS
Olaparib Impurity C Olaparib Impurity C
Olaparib Impurity F Olaparib Impurity F
Dapoxetine Impurity 4 Dapoxetine Impurity 4
Glycopyrrolate impurity 3 Glycopyrrolate impurity 3
Lamotrigine impurity B Lamotrigine impurity B
Ramosetron Impurity 3 Ramosetron Impurity 3
Loratadine Impurity 1 Loratadine Impurity 1 C22H23ClN2O2
Metoprolol IMpurity 6 Metoprolol IMpurity 6 C15H25NO3
Pitavastatin Impurity 5 Pitavastatin Impurity 5
Prasugrel Impurity C Prasugrel Impurity C
Trifluridine Impurity 2 Trifluridine Impurity 2
Cefazolin Impurity 4 Cefazolin Impurity 4
Vonoprazan Impurity 3 Vonoprazan Impurity 3
Cinacalcene Impurity I Cinacalcene Impurity I
Valsartan Impurity 2 Valsartan Impurity 2 C24H29N5O3
Istradefylline Impurity 2 Istradefylline Impurity 2
Etoricoxib Impurity 3 Etoricoxib Impurity 3
Apremilast Impurity 5 Apremilast Impurity 5 200710-04-9 C11H13NO4
Topiroxostat Imp.C Topiroxostat Imp.C
(3R,4S)-1-(4-chlorophenyl)-3-((S)-3-(4-fluorophenyl)-3-hydroxypropyl)-4-(4-hydroxyphenyl)azetidin-2-one (3R,4S)-1-(4-chlorophenyl)-3-((S)-3-(4-fluorophenyl)-3-hydroxypropyl)-4-(4-hydroxyphenyl)azetidin-2-one 1700622-08-7 C24H21ClFNO3
Rabeprazole Impurity 2 Rabeprazole Impurity 2 1163685-31-1 C11H15NO4
Regorafenib Impurity 8 Regorafenib Impurity 8
Flomoxef Impurity 5 Flomoxef Impurity 5 134237-56-2 C25H23ClF2N2O6S
Ranitidine  impurity Ranitidine impurity 94167-82-5 C10H18N2O
nicergoline impurity E nicergoline impurity E
Ethanesulfonic acid ethyl ester Ethanesulfonic acid ethyl ester 1912-30-7 C4H10O3S
DONEPEZIL HYDROCHLORIDE DONEPEZIL HYDROCHLORIDE 110119-84-1 C24H30ClNO3
(2S,5R)-3α-Chloromethyl-3-methyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2β-carboxylic acid benzhydryl ester (2S,5R)-3α-Chloromethyl-3-methyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2β-carboxylic acid benzhydryl ester 85573-73-5 C21H20ClNO3S
2-Propylidenepropanedinitrile 2-Propylidenepropanedinitrile 52833-34-8 C6H6N2
4,4'-Dihydroxyazoxybenzene 4,4'-Dihydroxyazoxybenzene 15596-57-3 C12H10N2O3
5-[[(2-Aminoethyl)thio]methyl]-N,N-dimethyl-2-furfurylamine 5-[[(2-Aminoethyl)thio]methyl]-N,N-dimethyl-2-furfurylamine 66356-53-4 C10H18N2OS
2-[2-(2-METHOXY-PHENOXY)-ETHYL]-ISOINDOLE-1,3-DIONE 2-[2-(2-METHOXY-PHENOXY)-ETHYL]-ISOINDOLE-1,3-DIONE 26646-63-9 C17H15NO4
(3AS,7AS)-HEXAHYDRO-1H-ISOINDOLE-1,3(2H)-DIONE (3AS,7AS)-HEXAHYDRO-1H-ISOINDOLE-1,3(2H)-DIONE 117307-09-2 C8H11NO2
N-(4-methoxy-2-(4-(3-methoxyazetidin-1-yl)piperidin-1-yl)-5-((6-(2- methyl-2H-indazol-5-yl)pyrimidin-4-yl)amino)phenyl)acrylamide N-(4-methoxy-2-(4-(3-methoxyazetidin-1-yl)piperidin-1-yl)-5-((6-(2- methyl-2H-indazol-5-yl)pyrimidin-4-yl)amino)phenyl)acrylamide
Amifostine Thiol Dihydrochloride Amifostine Thiol Dihydrochloride 14653-77-1 C5H14N2S
Butyl Itraconazole Butyl Itraconazole 89848-51-1 C35H38Cl2N8O4
Betamethasone 9,11-Epoxide Propionate Betamethasone 9,11-Epoxide Propionate 205105-83-5 C25H32O6
8-Methoxyquinolin-2(1H)-one 8-Methoxyquinolin-2(1H)-one 22614-69-3 C10H9NO2
5-Deshydroxymethyl-5-chloro Losartan  (Losartan Impurity K) 5-Deshydroxymethyl-5-chloro Losartan (Losartan Impurity K) 1159977-26-0 C21H20Cl2N6
Desfluoro Fluvoxamine Desfluoro Fluvoxamine 1217214-94-2 C15H22F2N2O2
Nilotinib N-Oxide Nilotinib N-Oxide 1246817-85-5 C28H22F3N7O2
Bis[(2-guanidino-4-thiazolyl)methyl]disulfide Bis[(2-guanidino-4-thiazolyl)methyl]disulfide 129083-44-9 C10H14N8S4
3-AMino-4-(2-chlorophenyl)-6-nitro-2(1H)-quinolinone 3-AMino-4-(2-chlorophenyl)-6-nitro-2(1H)-quinolinone 55198-89-5 C15H10ClN3O3
(E/Z)-FK-506 26,28-Allylic Ester RearrangeMent IMpurity (E/Z)-FK-506 26,28-Allylic Ester RearrangeMent IMpurity 131944-48-4 C44H69NO12
[[4-[(3-Methylphenyl)aMino]-3-pyridinyl]sulfonyl]carbaMic Acid Ethyl Ester [[4-[(3-Methylphenyl)aMino]-3-pyridinyl]sulfonyl]carbaMic Acid Ethyl Ester 72810-57-2 C15H17N3O4S
ethyl(2R,4S)-5-([1,1'-biphenyl]-4-yl)-4-(4-ethoxy-4-oxobutanamido)-2-methylpentanoate ethyl(2R,4S)-5-([1,1'-biphenyl]-4-yl)-4-(4-ethoxy-4-oxobutanamido)-2-methylpentanoate C26H33NO5
Tacrolimus Impurity C Tacrolimus Impurity C
Levodopa impurity 5 Levodopa impurity 5
(R)-N-(5-((R)-2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidin-3-yl)-3-hydroxypyrrolidine-1-carboxamide (R)-N-(5-((R)-2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidin-3-yl)-3-hydroxypyrrolidine-1-carboxamide 1223404-68-9 C21H22F2N6O2
Afatinib Impurity 18 Afatinib Impurity 18 2316847-95-5 C22H20ClFN4O4
Ivabradine Impurity 1 Hydrochloride Ivabradine Impurity 1 Hydrochloride 85175-52-6 C16H25ClN2O3
SofosBuvir Impurity 3 SofosBuvir Impurity 3
phenyl (4-((6-carbamoyl-7-methoxyquinolin-4-yl)oxy)-2-chlorophenyl)carbamate phenyl (4-((6-carbamoyl-7-methoxyquinolin-4-yl)oxy)-2-chlorophenyl)carbamate 417722-95-3 C24H18ClN3O5
Canagliflozin Impurity 10 Canagliflozin Impurity 10 1672658-93-3 C24H25FO6S
Canagliflozin Impurity F Canagliflozin Impurity F
Empagliflozin impurity E Empagliflozin impurity E
Fasudil Impurity 6 Fasudil Impurity 6 2044706-58-1 C11H11NO3S
Ibrutinib Impurity 11 Ibrutinib Impurity 11 C27H31N7O2
Nintedanib impurity I Nintedanib impurity I 334951-61-0 C30H31N5O4
(Z)-3-(hydroxy(phenyl)methylene)-2-oxoindoline-6-carboxylic acid compound with 3-benzoyl-2-oxoindoline-6-carboxylic acid (Z)-3-(hydroxy(phenyl)methylene)-2-oxoindoline-6-carboxylic acid compound with 3-benzoyl-2-oxoindoline-6-carboxylic acid 1253768-91-0 C16H11NO4
Everolimus Related Compound 3 Everolimus Related Compound 3 147438-30-0 C32H49NO9
(R)-isopropyl 2-(((R)-(perfluorophenoxy)(phenoxy)phosphoryl)amino)propanoate (R)-isopropyl 2-(((R)-(perfluorophenoxy)(phenoxy)phosphoryl)amino)propanoate 1627824-09-2 C18H17F5NO5P
Cefuroxime Sodium EP Impurity C Cefuroxime Sodium EP Impurity C 69822-88-4 C15H15N3O6S
Acemetacin EP Impurity F/Carboxymethyl Acemetacin/[[[[[1-(4-Chlorobenzoyl)-5-methoxy-2-methyl-1H-indol-3-yl]acetyl]oxy]acetyl]oxy]acetic acid Acemetacin EP Impurity F/Carboxymethyl Acemetacin/[[[[[1-(4-Chlorobenzoyl)-5-methoxy-2-methyl-1H-indol-3-yl]acetyl]oxy]acetyl]oxy]acetic acid 76812-49-2 C23H20ClNO8
Cefuroxime Sodium EP Impurity G Cefuroxime Sodium EP Impurity G 97232-98-9 C17H17N3O8S
2-chloro-4-(cyclopentylamino)-5-pyrimidinyl ethanone 2-chloro-4-(cyclopentylamino)-5-pyrimidinyl ethanone 1244949-62-9 C11H14ClN3O
Cetirizine 3-Chloro Impurity Cetirizine 3-Chloro Impurity 1232460-31-9 C21H25ClN2O3
Donepezil Impurity 8 Donepezil Impurity 8 1808997-65-0 C18H23NO4
Rivaroxaban Impurity 23 Rivaroxaban Impurity 23
(Z)-2-(2-aminothiazol-4-yl)-2-(hydroxyimino)acetamide (Z)-2-(2-aminothiazol-4-yl)-2-(hydroxyimino)acetamide 1450758-21-0 C5H6N4O2S
Fosfomycin Trometamol impurity C Fosfomycin Trometamol impurity C 23001-39-0 C4H12NO6P
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