| Company Name: |
Mainchem Co., Ltd.
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| Tel: |
+86-0592-6210733 |
| Email: |
sale@mainchem.com |
| Products Intro: |
Product Name:HYDROETHIDINE
CAS:38483-26-0
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| Company Name: |
Wuhan EnkiLife Biotech Co., Ltd. Gold
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| Tel: |
027-87002838 13212728378 |
| Email: |
order@enkilife.cn |
| Products Intro: |
Product Name:Dihydroethidium (Hydroethidine;DHE)
Package:1支
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| | HYDROETHIDINE Basic information |
| | HYDROETHIDINE Chemical Properties |
| Boiling point | 444.96°C (rough estimate) | | density | 1.1227 (rough estimate) | | refractive index | 1.6380 (estimate) | | storage temp. | −20°C | | solubility | acetonitrile: soluble | | form | solid | | color | pink | | Appearance | Solid Powder | | biological source | goat | | CAS DataBase Reference | 38483-26-0(CAS DataBase Reference) |
| Hazard Codes | Xn | | Risk Statements | 36/37/38-22 | | Safety Statements | 37/39-26 | | WGK Germany | 3 | | F | 8-10 | | Storage Class | 13 - Non Combustible Solids |
| | HYDROETHIDINE Usage And Synthesis |
| Description | Dihydroethidium is a cell-permeable blue fluorescent dye, which intercalates with nucleic acids and emits a red fluorescence detectable qualitatively by fluorescent microscopy or quantitatively by HPLC. It is a superoxide indicator. It exhibits blue fluorescence in the cytosol until oxidizedto ethidium, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red. It is neuroprotective by reducing superoxide in mice after stroke. It has been used to detect reactive oxygen species during the phagocytic respiratory burst and for the detection of intracellular superoxide in cultured cells. | | Uses | Dihydroethidium, also known as hydroethidine, is a dye that can permeate viable cells and accumulate in the nucleus after it is dehydrogenated to ethidium bromide and is used to measure oxygen reduction in phagocytes during respiratory burst. In the presence of an oxidant it will fluoresce and has been used as a dye in flow cytometry. Studies have used Dihydroethidium to study alveolar macrophages, blood leukocytes, and epithelial cells, among others, and has be used to measure intracellular H2O2 and O2-, and reactive oxygen. Dyes and metabolites. | | General Description | A cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry. | | Biochem/physiol Actions | Cell permeable: yes | | Excitation / Emission Maximum | 490 nm/590 nm |
| | HYDROETHIDINE Preparation Products And Raw materials |
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