| Company Name: |
Nanjing Dulai Biotechnology Co., Ltd.
|
| Tel: |
025-84699383-8003;025-846993838003-8003 18013301593;18013301590 |
| Email: |
njduly@126.com |
| Products Intro: |
Product Name:Fumarase
CAS:9032-88-6
Purity:>=20U/mg solid Package:5ku
|
| Company Name: |
Sigma-Aldrich
|
| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Fumarase from porcine heart
CAS:9032-88-6
Purity:ammonium sulfate suspension, 300-500 units/mg protein (biure Package:5KU Remarks:F1757-5KU
|
|
| Product Name: | EC 4.2.1.2 | | Synonyms: | L-MALATE HYDROLYASE;FUMARASE;FUMARATE HYDRATASE;EC 4.2.1.2;fumarase from porcine heart;fumarase from rabbit liver;Hydratase, fumarate;FUMARASE PRESTAINED FROM PORCINE HEART | | CAS: | 9032-88-6 | | MF: | | | MW: | 0 | | EINECS: | 232-887-7 | | Product Categories: | Elisa Kit-plant ELISA Kit | | Mol File: | Mol File | ![EC 4.2.1.2 Structure]() |
| | EC 4.2.1.2 Chemical Properties |
| storage temp. | 2-8°C | | form | ammonium sulfate suspension | | Specific Activity | ≥300units/mg protein (biuret) | | EPA Substance Registry System | Hydratase, fumarate (9032-88-6) |
| WGK Germany | 3 | | TSCA | TSCA listed | | Storage Class | 10 - Combustible liquids | | Hazard Classifications | Skin Sens. 1 |
| | EC 4.2.1.2 Usage And Synthesis |
| Uses | Fumarase from porcine heart has been used:
- as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
- for immobilized enzyme based stability assay
- in fumarate hydratase assays in human lung carcinoma cells (A549)
| | Definition | An enzyme that catalyzes the interconversion of
fumaric acid and malic acid. It is an important reactant in the tricarboxylic acid cycle. | | General Description | Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes. | | Biochem/physiol Actions | Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism. | | Description | Fumarase Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 467 amino acids (44-510) and having a molecular mass of 50.2 kDa.
Fumarate Hydratase is purified by proprietary chromatographic techniques. | | Source | Escherichia Coli | | Background | Fumarase is an enzymatic factor of Krebs cycle, which catalyzes the formation of L-malate from fumarate. Fumarase exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is aimed to the mitochondrion, where the removal of the extension results in the same form as in the cytoplasm. Fumarase is similar to a number of thermostable Class-2 fumarases and functions as a homotetramer. Mutations in the Fumarase gene causes fumarase deficiency and leads to progressive encephalopathy, cerebral atrophy and developmental delay. Fumarase enzyme is also thought to act as a tumor suppressor. Leydig cell tumors are caused by Fumarase mutations and represents one of the first reports of germline mutations in any type of adult testicular tumor. |
| | EC 4.2.1.2 Preparation Products And Raw materials |
|