4-Methylumbelliferyl-beta-D-glucuronide Cas 6160-80-1

Product Description

Product Name: 4-Methylumbelliferyl-beta-D-glucuronide

Synonyms: 4-methylumberiferryl-beta-D-glucuronide trihydrate

CAS No. 6160-80-1

M.F.: C16H16O9

M.W.: 352.3

EINECS: 228-186-0

Specifications

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4-Methylumbelliferyl-β-D-glucuronide (MUG) is a synthetic, fluorogenic enzyme substrate. It consists of two parts:

• 4-Methylumbelliferone (4-MU): A fluorescent blue molecule.

  β-D-Glucuronic Acid: A sugar acid.

These two parts are linked by a beta-glycosidic bond. The key property is that MUG itself is non-fluorescent. However, when the enzyme β-glucuronidase (GUS) cleaves this bond, it releases the highly fluorescent 4-methylumbelliferone. This reaction allows for extremely sensitive detection and quantification of the enzyme's activity.

2. Primary Uses in "Chemical Grade"

"Chemical grade" or "reagent grade" MUG is supplied with high purity and detailed specifications for scientific and diagnostic applications, not for therapeutic use. Its uses are almost exclusively in laboratories:

a. Microbiology & Water Safety Testing

• Principle: The enzyme β-glucuronidase is a key marker for Escherichia coli (E. coli).

  Application: MUG is incorporated into culture media (as in the Colilert? test). If E. coli is present, its GUS enzyme hydrolyzes MUG, producing a fluorescent glow under UV light. This provides a rapid, specific, and sensitive method for detecting fecal contamination in water, food, and environmental samples.

b. Molecular & Cell Biology (GUS Reporter Assay)

• Principle: The gusA gene, which codes for β-glucuronidase, is a widely used reporter gene in genetic engineering.

  Application: Researchers fuse the gusA gene to a promoter of interest (e.g., from a plant gene) and introduce it into cells (commonly plants). When the promoter is active, GUS is produced. Adding MUG to tissue extracts or incubating whole tissues with it allows visualization and quantification of gene expression through fluorescence. For histology, a similar substrate (X-Gluc) that yields a blue precipitate is often used.

c. Biochemical & Pharmacological Research

• Principle: β-glucuronidase activity is involved in drug metabolism, bacterial pathogenesis, and lysosomal function.

  Application: Chemical-grade MUG is used as a standardized substrate to:

• Measure enzyme kinetics and inhibition.

  Study lysosomal storage disorders.

  Screen for inhibitors that could serve as new therapeutics.

  Investigate the metabolism of drugs that form glucuronide conjugates.

d. Diagnostic Enzymology

• Principle: Abnormal levels of β-glucuronidase can indicate certain diseases.

  Application: MUG serves as the core substrate in clinical chemistry assays to measure GUS activity in human serum or tissues, which can be a biomarker for conditions like certain cancers or liver diseases.

Key Characteristics of Chemical Grade MUG

• High Purity (e.g., ≥99% by HPLC): Essential for consistent, reproducible results and to avoid background fluorescence.

  Defined Specifications: Includes data on extinction coefficient, fluorescence properties, and limits for impurities.

  Packaging: Labeled "For research use only" or "For in vitro diagnostic use," stored to ensure stability (often desiccated, frozen, and protected from light).

  Supplier: Sold by fine chemical and biochemical suppliers (e.g., Sigma-Aldrich, Thermo Fisher, Toronto Research Chemicals).

Summary

Chemical-grade 4-Methylumbelliferyl-β-D-glucuronide is a critical fluorogenic reagent. Its sole purpose is to act as a precise detection tool for the enzyme β-glucuronidase. This makes it indispensable in water microbiology for E. coli detection, molecular biology as a reporter assay, and biochemical research for studying enzyme function and disease mechanisms.

Packing & Shipping

FAQ

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2. Could you please provide some samples?

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3. How should I pay?

We accept various payment terms such as L/C,T/T, Western Union and Money Gram.

4. Is there a discount?

Different quantity has different discount.

5.What's your MOQ?

Different products have different MOQ, such as:

1).Peptide products, the MOQ is 1 gram level

2).Liquid products, the MOQ is usually 50-100ML

3).API,  the MOQ is 1KG

4).Intermediate products and conventional product, the MOQ is 1KG; custom-tailored products, the MOQ depends on the production process.

5).Food additives and vitamin, the MOQ is usually one package: such as Vitamin C is 25KG / drum, Vitamin E oil or Vitamin A oil  is 5KG/bag;Vitamin E powder or Vitamin A powder is 20KG / cartons.

6).Agrochemical , the MOQ is 1KG, except preparations.

6.How do we take the quality complaint ?

First of all, our quality control will reduce the quality issue to near zero. If there is a quality problem caused by us, we will send you free goods for replacement or compensate your loss.

Company Information

Company profile

Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical & its intermediates, Food/Feed additives, Fine chemicals in distributing the products of our own company and affiliated enterprises, are the members of Hubei E-commerce Chamber.

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients’ requirement.

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves. 

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.

We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward powder, their satisfaction is our struggling goal.

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