5-ROX, SE NEW
| Price | $105 | $213 | $293 |
| Package | 1mg | 5mg | 10mg |
| Supply Ability: | 10g |
| Update Time: | 2026-05-23 |
Product Details
| Product Name: 5-ROX, SE | CAS No.: 209734-74-7 |
| Purity: 95.00% | Supply Ability: 10g |
| Release date: 2026/05/23 |
Product Introduction
Bioactivity
| Name | 5-ROX, SE |
| Description | 5-ROX, SE ([5-Carboxy-X-rhodamine, succinimidyl ester] *Single isomer*) is a labeling reagen used in the preparation of charge-modified dye-labeled Dideoxynucleotide Triphosphates (ddNTP) to "direct-load" DNA sequencing. |
| Cell Research | Instructions I. Solution preparation 1. Storage solution preparation: 5-ROX, SE are dissolved in anhydrous DMSO or DMF. The concentration is generally recommended to be 1-10 mM. The specific concentration can be adjusted according to the experimental needs. 2. Working solution preparation: Use water or PBS to dilute the storage solution to a certain concentration. The specific concentration needs to be determined according to the experimental conditions. II. Operation steps 1. Labeling reaction: 1) Mix the dissolved 5-ROX, SE with the amine-containing ddNTP solution. 2) The reaction is usually carried out at pH 8.0-9.0 to promote the reaction of NHS ester with the amine group in the ddNTP molecule. 3) The reaction time is usually 30 minutes to 1 hour. The reaction can be carried out at room temperature or overnight at 4°C. The specific reaction conditions are optimized according to the requirements of the experiment. 2. Removal of unreacted dye: 1) After the reaction is completed, use dialysis, gel filtration or centrifugation to remove the unreacted dye to avoid affecting the subsequent sequencing reaction. 2) These steps help ensure pure labeled ddNTPs for DNA sequencing. 3. DNA sequencing using labeled ddNTPs: 1) Use 5-ROX, SE labeled ddNTPs for DNA synthesis reactions. This method allows for direct loading of fluorescent labels during DNA synthesis for different base recognition. 2) The fluorescence properties of 5-ROX (Ex/Em: ~570/602 nm) allow it to be well distinguished from other commonly used fluorescent labels in sequencing, helping to identify different bases. Notes: 1. Labeled ddNTPs should be stored at -20°C to avoid repeated freezing and thawing to ensure label stability. 2. Unused 5-ROX, SE solutions should be kept at -20°C and avoid exposure to strong light to avoid fluorescence decay. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Storage | Store at low temperature,Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : Slightly soluble |
| Keywords | Inhibitor | inhibit | DNA sequencing | 5-ROX, SE | 5ROX, SE | 5-Carboxy-X-rhodamine, succinimidyl ester | 5 ROX, SE |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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