The main application fields of 5,6-Dihydroxyindole are as follows:

It is a key intermediate in melanin synthesis and plays an important role in research on the treatment of hypopigmentary diseases such as vitiligo. Meanwhile, it possesses certain antioxidant and neuroprotective properties, and may have potential applications in the treatment of neurodegenerative diseases.

It can be used in hair dye products as a natural hair coloring ingredient. Compared with traditional chemical hair dyes, it causes less damage to hair and scalp and can provide a more natural color. In addition, due to its association with melanin synthesis, it can also be used in the research and development of some skin-lightening and spot-removing products to improve skin tone by regulating melanin metabolism.

It can serve as a precursor for functional materials, and is used in the preparation of materials with special properties such as polymers and thin films.