Product Details
| Product Name:
Azure B |
CAS No.:
531-55-5 |
| Supply Ability:
10g |
Release date:
2026/05/20 |
Product Introduction
Bioactivity
| Name | Azure B |
| Description | Azure B (Azure B chloride) is a cationic dye and the major metabolite of Methylene blue. Azure B is a selective, and reversible inhibitor of MAO-A (IC50s: 11 and 968 nM for recombinant human MAO-A and MAO-B). |
| Cell Research | Instructions
1. Solution configuration
1. Configuration of mother liquor: Use sterile water or PBS to configure MHI-148 into 1-10mM mother liquor and store it at -20℃ or -80℃. Please aliquot it to avoid repeated freeze-thawing.
2. Configuration of working fluid: When in use, dilute the MHI-148 mother liquor into a working fluid that meets the requirements and adjust according to the experimental requirements.
2. Blood smear staining:
1. Prepare an Azure B staining solution, usually by dissolving Azure B chloride in buffer or solvent.
2. Apply the staining solution to the prepared blood smear.
3. Allow the smear to incubate in solution for a few minutes to promote cell staining.
4. Rinse the smear thoroughly with water to remove excess dye and then air dry.
5. Check the stained smear under a microscope and analyze the morphology of blood cells.
3. Monoamine oxidase (MAO) inhibition:
1. Introduce Azure B into in vitro experiments, the inhibitory effect is usually determined using recombinant human MAO-A and MAO-B.
2. The IC50 value at a specific experimental setting can be determined through dose response studies.
3. In addition, the antidepressant effect of Azure B can be evaluated in animal models through behavioral experiments (such as forced swimming experiments, tail suspension experiments).
The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| In vivo | Azure B (4-30 mg/kg; i.p.; once) reduces immobility in the forced swim test [1]. |
| Storage | Keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : Insoluble
|
| Keywords | stains | reversible | MonoamineOxidase | Monoamine Oxidase | MAO-B | MAO-A | dye | Azure B | antidepressant-like |
| Inhibitors Related | Safinamide | 1,4-Naphthoquinone | 4-Chlorochalcone | Paeonol | Hydroxylamine hydrochloride | Isatin | Methylene Blue trihydrate | β-Aminopropionitrile | Aminoacetone hydrochloride | Allylthiourea | D-(-)-Quinic acid | Amitraz |
| Related Compound Libraries | Highly Selective Inhibitor Library | NO PAINS Compound Library | Anti-Aging Compound Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
Recommended supplier
| Product name |
Price |
|
Suppliers |
Update time |
|
|
|
VIP3Y
|
Henan Fengda Chemical Co., Ltd
|
2025-09-25 |
|
|
|
|
Hebei Chuanghai Biotechnology Co., Ltd
|
2025-06-27 |
|
|
$8.00
|
VIP8Y
|
Career Henan Chemical Co
|
2019-07-06 |
United States