Bromobimane NEW
| Price | $133 |
| Package | 50mg |
| Supply Ability: | 10g |
| Update Time: | 2026-06-02 |
Product Details
| Product Name: Bromobimane | CAS No.: 71418-44-5 |
| Purity: 98.27% | Supply Ability: 10g |
| Release date: 2026/06/02 |
Product Introduction
Bioactivity
| Name | Bromobimane |
| Description | Bromobimane (Monobromobimane) is a non-fluorescent substance, but reacts with thiols to produce a fluorescent product.Bromobimane is used as a probe, often in clinical medicine, to test for sulfide levels in blood. |
| Cell Research | Instructions I. Solution preparation: 1. Mother solution preparation: Dissolve Bromobimane in an appropriate solvent, such as anhydrous DMSO, DMF or methanol, to prepare a stock solution of the required concentration. 2. Working solution preparation: The recommended concentration of the stock solution is 100 mM for subsequent dilution. Dilute the stock solution to the working concentration, usually 10-50 μM. II. Operation steps Cell staining 1. Grow cells to an appropriate density; wash cells with PBS and remove the culture medium. 2. Add the diluted Bromobimane solution to the cells and incubate at 37°C in the dark for 15-30 minutes. 3. After incubation, wash the cells 3 times with PBS to remove unbound dye. 4. Fluorescence detection: Use a fluorescence microscope or flow cytometer for detection. The excitation wavelength of Bromobimane is about 390 nm and the emission wavelength is about 478 nm. Precautions: 1) Wear gloves during operation to avoid contact between skin or mucous membranes and reagents. 2) Avoid light exposure during incubation and storage to prevent fluorescence quenching. 3) After staining, fluorescence detection and analysis should be performed immediately. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| In vitro | Bromobimanes in solution (aqueous or organic solvents of medium polarity) react with small thiols, and with reactive protein thiol groups (e.g., hemoglobin). The reactions of bromobimanes with thiols are second-order and dependent on pH, the active nucleophile being the thiolate anion. The reaction of bromobimane with a thiolate converts the nonfluorescent agent into water-soluble fluorescent products [1]. The highly selective, rapid reactivity of bromobimanes toward thiols, the stability and fluorescence yield of the thiol derivatives, the ease of separation of the derivatives by reversed-phase HPLC, and the availability of both cell-penetrating and nonpenetrating forms, make the use bromobimanes an extremely powerful approach to the analysis of low molecular weight biothiols [2]. |
| Storage | Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween-80+45% Saline : 2.5 mg/mL (9.22 mM), Sonication is recommended. 10% DMSO+90% Saline : 10 mg/mL (36.89 mM), Solution. DMSO : 125 mg/mL (461.07 mM), Sonication is recommended. |
| Keywords | Dye reagent | Bromobimane |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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