Coelenterazine h NEW
| Price | $89 | $228 | $372 |
| Package | 1mg | 5mg | 10mg |
| Supply Ability: | 10g |
| Update Time: | 2026-07-15 |
Product Details
| Product Name: Coelenterazine h | CAS No.: 50909-86-9 |
| Purity: 99.58% | Supply Ability: 10g |
| Release date: 2026/07/15 |
Product Introduction
Bioactivity
| Name | Coelenterazine h |
| Description | Coelenterazine h is a dehydroxy derivative of natural coelenterazine and serves as a luminescent substrate for RLuc8. Coelenterazine h is Ca2?-sensitive, making it an effective tool for measuring minute changes in Ca2? concentration. Coelenterazine h is widely used in cell biology, molecular biology, drug discovery, and in vivo imaging. |
| In vitro | Methods: In BRET sensor (BRAC) studies, Coelenterazine-h (20 μM in vitro, 10 μM in cells) serves as the substrate for RLuc8, initiating the BRET signaling chain for real-time monitoring of Ca2? binding kinetics. Changes in Venus (530 nm) emission were recorded at a high frequency of 1 kHz via stop-and-go spectroscopy. Results: The Ca2? dissociation time constant τ_off = 0.21 s was successfully resolved, approximately 14 times faster than conventional FRET indicators. [1] Methods: C6 (rat glioma) cells were transfected with pCMV-Rluc plasmid. Cell lysates were obtained 48 hours post-transfection. Cell lysates were mixed with Coelenterazine h (0.1, 1, 10, 50, 100, 200 μg/mL) and measured for 10 seconds. A luminometer recorded RLU/mg protein. Results: Luminescence increased with Coelenterazine h concentration, peaking at 50 μg/mL, then decreased at higher doses. [2] Methods: Human breast cancer cells (MCF-7) were treated with Rluc8.6-KR-LP (10 μM) for 24 hours, followed by Coelenterazine h (150 μM) for an additional 24 hours. Analysis included fluorescence microscopy (DAPI staining for live cells, SYTOX Green for dead cells, KR red fluorescence tracing) and flow cytometry quantification. Results: The Rluc8.6-KR-LP + Coelenterazine H group exhibited substantial cell death, with KR red fluorescence localized to the cell membrane. [3] |
| In vivo | Methods: C6-Rluc cells (1×10?) were injected into the mouse tail vein (cells naturally homed to liver/lung). Coelenterazine h (2.8 mg/kg) was administered via tail vein injection 90 minutes after cell injection. Imaging was performed using a cooled CCD camera for 7-10 minutes. Following euthanasia, tissue homogenates were collected and verified using a luminometer. Results: Signals were detectable in the chest and upper abdomen (liver/lung regions), peaking at 5 minutes and decaying between 7–10 minutes. Tissue homogenate analysis confirmed signals primarily originated from the lungs. [2] Methods: NOD SCID-γ immunodeficient mice subcutaneously implanted with MDA-MB-231 human breast cancer cells. Three intra-tumoral injections of Rluc8.6-KR-LP (1 nmol) were administered via intratumoral injection. Coelenterazine h (5 μg) was injected subcutaneously on the second day after protein injection. Tumor volume was measured 2-3 times weekly. Results: Rluc8.6-KR-LP + Coelenterazine h significantly inhibited tumor growth, with an average volume of 127 mm3 on day 34.[3] |
| Storage | Keep away from direct sunlight,Keep away from moisture,The compound is unstable in solution. Please use soon, Powder: -20°C for 3 years Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 237.5 mg/mL (582.88 mM), Sonication is recommended. |
| Keywords | Inhibitor | inhibit | Coelenterazine h |
| Inhibitors Related | Quadrol | Tricaine methanesulfonate | Nisoldipine | 2,4,6-Tri-tert-butylphenol | Chlorocresol | L-Ascorbic acid | L-Phenylalanine | L-Ascorbic acid sodium salt | 2-Nitrobenzoic acid | Magnesium Chloride Hexahydrate | Magnesium sulfate | Cinchonine |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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- Address: 36 Washington Street, Wellesley Hill, MA
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