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Postion:Product Catalog >Biologics>Dyes/Staining Agents>Cy5-N3
Cy5-N3
  • Cy5-N3

Cy5-N3 NEW

Price $112 $266 $413
Package 1mg 5mg 10mg
Supply Ability: 10g
Update Time: 2026-05-11

Product Details

Product Name: Cy5-N3 CAS No.: 1621101-43-6
Purity: 98.98% Supply Ability: 10g
Release date: 2026/05/11

Product Introduction

Bioactivity

NameCy5-N3
DescriptionCY5-N3 (Sulfo-Cyanine5-azide) is an anthocyanin fluorescent dye with azide groups, utilized for cell imaging, tissue imaging, and in vivo imaging.
Cell ResearchInstructions I. Solution preparation 1. Stock solution: Dissolve CY5-N3 in DMSO, DMF or appropriate buffer. Common stock solution concentration: 1–10 mM. Store at -20°C in the dark to prevent photodegradation and degradation. 2. Working solution: Dilute the stock solution to the desired concentration (e.g. 0.1–10 μM) with an appropriate buffer (e.g. PBS or HEPES buffer, pH 7.2–7.4). Prepare fresh to ensure the best results. II. Bioorthogonal labeling using CY5-N3 1. SPAAC labeling protocol (copper-free click chemistry): Prepare functionalized target biomolecules (e.g. DBCO-modified proteins, peptides or nucleic acids). Mix CY5-N3 with the target molecule at the desired concentration (e.g. 1–10 μM) and incubate in a reaction buffer (e.g. PBS, pH 7.4). Usually incubate for 30 minutes to 2 hours at room temperature. 2. CuAAC labeling protocol (copper-catalyzed click chemistry): 1) Add a small amount of copper (such as CuSO? and a reducing agent such as ascorbic acid) to the reaction system. 2) Mix CY5-N3 with a biomolecule with an alkyne group and place it in a reaction buffer (such as PBS or Tris-HCl, pH 7.4). 3) Incubate at room temperature for 30–60 minutes with appropriate stirring. 4) Purify the labeled biomolecule using molecular sieve chromatography, ultrafiltration or similar methods to remove unreacted dye. 3. Imaging applications Cell imaging: (1) Label live or fixed cells by using CY5-N3-labeled biomolecules or directly in SPAAC/CuAAC labeling. (2) Wash cells with PBS to remove unbound dye. (3) Analyze the fluorescence signal using a confocal microscope or fluorescence microscope, and select a filter set suitable for CY5 (excitation wavelength: approximately 646 nm, emission wavelength: approximately 662 nm). Tissue imaging: (1) Stain tissue sections with CY5-N3-labeled probes. (2) Wash sections thoroughly to remove background fluorescence. (3) Analyze using a fluorescence microscope or whole-slice imaging system. In vivo imaging: (1) Inject CY5-N3-labeled probes into animal models. (2) Perform near-infrared fluorescence imaging using an in vivo imaging system (such as IVIS) or similar platform. (3) Monitor the distribution, targeting, and clearance of labeled probes. Precautions 1. Photosensitivity: CY5-N3 is light-sensitive. Avoid long-term exposure to light and store in a dark place. 2. Reaction optimization: Optimize dye concentration, reaction time, and buffer conditions to achieve efficient labeling and reduce background. 3. Copper toxicity (for CuAAC): Copper ions may affect biological samples, so the copper concentration should be carefully controlled, or SPAAC should be selected for copper toxicity issues. 4. Stability: The stock solution should be aliquoted to avoid repeated freezing and thawing to maintain the activity of the solution. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
StorageStore at low temperature,Keep away from direct sunlight,Keep away from moisture,The compound is unstable in solution. Please use soon Powder: -20°C for 3 years Shipping with blue ice/Shipping at ambient temperature.
Solubility InformationDMSO : 20 mg/mL (27.07 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween-80+45% Saline : 2 mg/mL (2.71 mM), Sonication is recommended.
KeywordsCY5-N3
Inhibitors RelatedStavudine | Aceglutamide | Hemin | Tamoxifen | Cysteamine hydrochloride | Guanidine hydrochloride | Hydroxychloroquine | Enzalutamide | Paeonol | Naringin | Alginic acid | Sildenafil citrate

Company Profile Introduction

Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers. TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.

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TargetMol Chemicals Inc.

4YR United StatesUnited States
  • Since: 2011-01-07
  • Address: 36 Washington Street, Wellesley Hill, USA
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