DAF-FM DA NEW
| Price | Get Latest Price |
| Package | 1removed |
| Supply Ability: | 10g |
| Update Time: | 2026-07-22 |
Product Details
| Product Name: DAF-FM DA | CAS No.: 254109-22-3 |
| Supply Ability: 10g | Release date: 2026/07/22 |
Product Introduction
Bioactivity
| Name | DAF-FM DA |
| Description | DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm). |
| Cell Research | Instructions: I. Solution preparation 1. Preparation of mother solution: Dissolve DAF-FM DA in DMSO to prepare a 10mM mother solution for standby use. 2. Preparation of working solution: Dilute the DAF-FM DA mother solution with PBS/H2O/DMEM to prepare a working solution for standby use. The commonly used concentration is 5-10μM. II. Operation steps 1. Pre-incubate BCa cells with 5 μM DAF-FM DA for 30 minutes to load the probe. 2. Subsequently, incubate the cells with fresh Hank's buffer for 20 minutes to complete deesterification. The DAF-FM DA probe can quantify the level of nitric oxide (NO) in cells. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| In vitro | BCa cells were pre-incubated with 5 μM DAF-FM DA for 30 minutes to load the probe. The DAF-FM DA probe quantifies nitric oxide (NO) levels in cells. [1] |
| In vivo | Using zebrafish embryo sections live-stained with DAF-FM DA (5 μM), it was confirmed that the fluorescent signal was primarily localized to areas of ongoing bone formation.[2] |
| Storage | Store at low temperature,Keep away from direct sunlight, Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Keywords | Diaminofluorescein-FM | DAF-FM DA | DAFFM DA | DAF FM DA |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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