Diethyl pyrocarbonate is an organic ester compound, which can be used as a modifying agent for His and Tyr residues in proteins; Durable probes used for structural division in dsDNA react partially or completely with non stacking bases; It can be used as antibacterial additive, ribonuclease inhibitor, histidine residue modifier, and reagent for converting imine to carbamate. Diethyl pyrocarbonate can be used to extract RNA in biological experiments. It is an ester compound, which can be used as a modifying agent of diethyl pyrocarbonate protein histidine and tyrosine, and also a chemical modifier of RNA enzyme. It reacts with the imidazole ring of histidine, the active group of RNA enzyme, to inhibit the activity of RNA enzyme.