FITC NEW
| Price | $42 |
| Package | 100mg |
| Supply Ability: | 10g |
| Update Time: | 2026-05-15 |
Product Details
| Product Name: FITC | CAS No.: 3326-32-7 |
| Purity: 98.69% | Supply Ability: 10g |
| Release date: 2026/05/15 |
Product Introduction
Bioactivity
| Name | FITC |
| Description | FITC is a green fluorescein derivative. FITC is commonly used to label biomolecules such as antibodies, proteins, and nucleic acids. It can serve as a fluorescent probe for detecting cell viability, apoptosis, and other cellular processes. FITC has a maximum excitation wavelength of 494 nm and emits yellow-green fluorescence at its maximum emission wavelength of 520 nm. |
| Cell Research | I. Amine labeling 1. Binding: Dilute FITC in an appropriate solvent (usually dimethylsulfonamide DMSO) and mix it with the target biomolecule solution. The reaction is usually carried out under slightly alkaline conditions (pH 8.0-9.0) to ensure effective binding of FITC. 2. Incubation: Incubate the mixture at room temperature or 4°C for 1-2 hours. Since FITC is light-sensitive, it needs to be protected from light. 3. Purification: After the reaction is completed, excess FITC is removed by purification techniques such as dialysis and gel filtration to obtain labeled biomolecules. II. Cell imaging 1. Cell staining: Apply FITC-labeled antibodies or proteins to cells or tissues and incubate for a certain period of time (usually 30 minutes to 1 hour) at room temperature or 4°C. 2. Fluorescence microscopy: After washing away unbound antibodies, use a fluorescence microscope to observe cells or tissues. FITC can be excited at a wavelength of about 495 nm and emit at a wavelength of 519 nm. III. pH and Cu2+ sensitivity 1. pH sensitivity: When FITC-labeled molecules are placed in solutions of different pH values, the fluorescence intensity will change with the change of pH and can be quantitatively measured. 2. Cu2+ detection: The fluorescence of FITC is also affected by Cu2+, so it can be used to determine the concentration of Cu2+ in the sample by observing the change of fluorescence intensity. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| In vivo | Methods: P17 oxygen-induced retinal disease (OIR) mice received retro-orbital injections of FITC-dextran: 0.05 ml (50 mg/ml). Specimens were collected immediately post-injection. Fluorescence microscopy examined orbital tissues and retinas of the injected eye and contralateral eye. Results: FITC-dextran was absorbed systemically, as demonstrated by bilateral retinal visualization following entry into the bloodstream via the orbital venous sinus.[1] |
| Storage | Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 250 mg/mL (642.05 mM), Sonication is recommended. |
| Keywords | Inhibitor | inhibit | FITC |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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