Hoechst 33258 trihydrochloride NEW
| Price | $53 |
| Package | 50mg |
| Supply Ability: | 10g |
| Update Time: | 2026-05-11 |
Product Details
| Product Name: Hoechst 33258 trihydrochloride | CAS No.: 23491-45-4 |
| Purity: 99.47% | Supply Ability: 10g |
| Release date: 2026/05/11 |
Product Introduction
Bioactivity
| Name | Hoechst 33258 trihydrochloride |
| Description | Hoechst 33258 trihydrochloride (Bisbenzimide) is a benzimidazole anti-filarial agent. it is fluorescent when it binds to certain nucleotides in DNA, thus providing a tool for the study of DNA replication. it also interferes with mitosis. |
| Cell Research | Solution preparation a. Preparation of stock solution: Dissolve 10 mg in 5 mL DMSO; (It is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) b. Preparation of Hoechst working solution Dilute the stock solution and dissolve it in serum-free cell culture medium or PBS to obtain a Hoechst working solution with a final concentration of 10 μg/mL; (Select the appropriate working solution concentration according to experimental requirements and prepare it for immediate use) c. Cell staining 1. Suspended cells (6-well plate) a. Centrifuge at 1000 g for 3-5 minutes at 4℃, and then discard the supernatant. Wash twice with PBS for 5 minutes each. The cell density is 1×106/mL; b. Add 1 mL of working solution and incubate at room temperature for 3-10 minutes; c. Centrifuge at 400 g for 3-4 minutes at 4℃, and discard the supernatant; d. Wash twice with PBS, 5 minutes each time; e. Resuspend cells with serum-free cell culture medium or PBS. Observe with fluorescence microscope or flow cytometer. 2. Adherent cells a. Culture adherent cells on sterile coverslips; b. Remove coverslips from culture medium and aspirate excess culture medium; c. Add 100 μL working solution, shake gently to completely cover cells, and incubate at room temperature for 3-10 minutes; d. Wash twice with culture medium, 5 minutes each time. Observe with fluorescence microscope or flow cytometer. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Storage | Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 6.58 mg/mL (12.32 mM), Sonication is recommended. |
| Keywords | Parasite | Inhibitor | inhibit | Hoechst 33258 trihydrochloride | HOE-33258 | HOE33258 | H-33258 Trihydrochloride | H33258 Trihydrochloride | H-33258 | H33258 | H 33258 Trihydrochloride | H 33258 | bisBenzimide H-33258 Trihydrochloride | bisBenzimide H33258 Trihydrochloride | bisBenzimide H-33258 | bisBenzimide H33258 | bisBenzimide H 33258 Trihydrochloride | bisBenzimide H 33258 |
| Inhibitors Related | Flubendazole | Gum arabic | Kojic acid | Urethane | Hydroxychloroquine | Metronidazole | 2-Amino-2-methyl-1-propanol | Doxycycline | Fenpyroximate | Methylene Blue trihydrate | Coumaran | Benzimidazole |
| Related Compound Libraries | Anti-Parasitic Compound Library | Bioactive Compound Library | NO PAINS Compound Library | Bioactive Compounds Library Max | Anti-Infection Compound Library | Human Metabolite Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
You may like
Recommended supplier
| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $163.00 |
VIP4Y
|
TargetMol Chemicals Inc.
|
2026-06-12 | |
|
Sichuan Zhuoyu Yantang Technology Co., Ltd.
|
2025-08-08 | |||
|
VIP2Y
|
Shaanxi Xianhe Biotech Co., Ltd
|
2026-06-23 | ||
| $1.00 |
VIP8Y
|
Career Henan Chemical Co
|
2019-07-14 |
United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
INQUIRY