N-Nitroso Tranexamic Acid

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E-mail: anna@molcoo.com

Product Information

• Product Number: T048015

• English Name: N-Nitroso Tranexamic Acid

• English Alias: (1R,1'R,4r,4'r)-4,4'-((nitrosoazanediyl)bis(methylene))dicyclohexanecarboxylic acid

• CAS Number: None

• Molecular Formula: C??H??N?O?

• Molecular Weight: 326.39

Advantages

• Well-defined with distinct functional groups: Contains two cyclohexanecarboxylic acid units and nitrosoazanediyl (-N(NO)-) linked by bis(methylene) groups. Unlike tranexamic acid (antifibrinolytic with amino cyclohexanecarboxylic acid), its nitroso polarity, dicarboxyl hydrophilicity, and cyclohexane hydrophobicity create significant differences, enabling precise differentiation via HPLC/ion-exchange chromatography as a specific marker;

• High stability and traceability: Rigid cyclohexane structures and stability of carboxyl/C-N bonds ensure stability under dark, low-temperature conditions. As a byproduct from amino nitrosation during storage/degradation of tranexamic acid, it directly reflects amino reactivity and nitrite exposure risk, improving process tracing accuracy;

• High detection sensitivity: Conjugation between nitroso and carbon chains shows characteristic UV absorption (230-260nm), combined with m/z 327 [M+H]? enabling ppb-level analysis via LC-MS, compatible with antifibrinolytic nitroso impurity systems.

Applications

• Pharmaceutical quality control: Used as an impurity reference standard to quantify N-Nitroso Tranexamic Acid in APIs, ensuring compliance with genotoxic impurity limits in pharmaceutical standards;

• Stability studies: Monitoring impurity levels under varying conditions (pH, light) to assess nitrosation trends and support safety assurance over shelf life;

• Synthesis assessment: Evaluating purity of amino-containing intermediates in tranexamic acid synthesis to reduce nitrosation risk at the source.

Background Description

Research Status

• Analytical method validation: Developing HPLC assays with C18 columns for separation, achieving 0.1 ppb detection limits;

• Nitrosation mechanism: Studying impurity formation kinetics under varying nitrite concentration and pH to clarify primary amine-to-nitroso derivative conversion pathways;

• Control strategies: Exploring nitrosation inhibitors (e.g., ascorbic acid) to keep impurity levels below safety limits (<0.001%);

• Toxicity evaluation: Conducting in vitro genotoxicity tests (e.g., Ames assay) to assess hazards and support limit setting.

We can also customize related analogues and modified peptides including HPLC, MS, 1H-NMR, MS, HPLC, IR, UV, COA, MSDS.
This product is intended for laboratory use only!

WhatsAPP: +86 17386083646
E-mail: anna@molcoo.com